Isolation and Characterization of Enhanced Berberine From the Calli Cultures of Coscinium Fenestratum L. An Endangered Medicinal Liana of Western Ghat India

K. Talat¹*, G. L. D. Krupadanam² and S. Y. Anwar¹  

¹Department of Genetics, Osmania University, Hyderabad India.

²Natural Products Labotatory, Department of Chemistry, Osmania University, Hyderabad - 500 007 India.

Corresponding Author E-mail: talatkh@yahoo.co.in

ABSTRACT: Present study describes in detail isolation and characterization of berberine isolated from the calli cultures of an endangered medicinal plant Coscinium fenestratum. Petiole derived calli cultures were established in MS medium supplemented with 2,4-D and BAP. Air dried calli cultures were homogenized with mortar and pestle then pure methanol was used repeatedly to extract until all the brown color had been removed from the material. Methanol was evaporated and the concentrated extract was separated by preparative TLC by using ethyl acetate: methanol (7:3). The crude extract was loaded into a silica column and eluted with different fractions of solvents from increasing polarity. The yellow fluorescent fraction showed the presence of berberine by TLC, was collected and evaporated by dryness. Yellow colored powder thus obtained was identified by comparing Rf- values on TLC. NMR data of the compound obtained from the calli cultures of C.fenestratum explain the presence of 20 carbons with 18 Protons. Observed molecular weight is 336 and molecular formula C20H18NO4 indicated the compound as berberine which is similar to the authentic sample of berberine and it is about 18 folds higher compared to the petiole of intact plant is the first report of yielding maximum berberine content compare to earlier studies.

KEYWORDS: Coscinium fenestratum; Berberine; calli culture

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