Biochemical and Antimicrobial Studies of the Genus Jatropha Excisa

D. S. V. G. K. Kaladhar¹*, K. V. V. V. Satyanarayana² and I. Bhaskar Reddy³

¹Department of Biochemistry and  Bioinformatics, GITAM Institute of Science Gitam University India.

²Department of Chemistry, Gitam Institute of Science Gitam University India.

³Department of Biochemistry and Bioinformatics, GITAM Institute of Science, GITAM University India.

Corresponding Author E-mail: dkaladhar@gmail.com

ABSTRACT: Jatropha excisa is an ornamental plant, which is employed as a source of medicine with different antimicrobial activities and differentiation in organization of metabolites. Metabolite analysis of this plant was analyzed phytochemically and screened against different microorganisms responsible for various infections. Qualitative analysis of various primary and secondary metabolites was analyzed by Thin Layer Chromatography. Present report has shown more number of amino acids in wet leaves, carbohydrates in seeds and dry leaves, lipids in seeds and dry leaves, and secondary metabolites in flowers. The antimicrobial activity of the fresh methanolic leaf extract was tested against standard strains of bacteria and fungi using agar well diffusion method. Plant fresh methanolic leaf extract of 0.1mg/ml concentration produced different zone formation on Microorganisms. The zone formation was high in gram-negative bacteria such as Escherichia coli (16 mm), Micrococcus leuteus (15 mm) and Proteus mirabilis (14 mm) compared to the gram-positive bacteria such as Streptococcus faecalis (12 mm), Klebsiella pneumoniae (11 mm) and Bacillus subtilis (12 mm). The extracts were found to be effective against bacteria (11-16 mm) and yeast (15 mm) than multicellular, filamentous fungi such as Aspergillus niger and Penicillium chrysogenum (9-11mm).

KEYWORDS: Jatropha excisa; TLC; Metabolites Antimicrobial activity

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