Effect of Ph on Lipase Specific Activities of Thermophilic Fungi From City Waste of Bareilly

Shashi Yadav¹*,  Chandra Prabha¹,  A. K. Jaitly¹,  S. K. Gupta² and M. Bansal²

¹Department of Plant Science, M. J. P. Rohilkhand University, Bareilly - 243 005 India.

²R.V. Northland Institute, 18 km on Gaziabad - Bulandshahar G. T. Road, Dadri (Greater Noida Phase-II), G. B. Nagar (U.P.) - 203 207 India.

Corresponding Author E-mail: mayank_pharma@rediffmail.com

ABSTRACT: The lipase of the Sporotrichum thermophile, Aspergillus fumigatus are important and versatile enzymes that are mainly used in fat and oil modification due to their strong 1, 3- regiospecificty. In the present study, five samples were collected randomly from different sites of Bareilly city, Transport Nagar, Nakatiya, Barabazar, University campus, Railway junction. Bareilly city were primarily screened by sprinkling method and serial dilution techniques for isolation of lipase producing species of fungi from Bareilly city. Out of five samples collected two isolates were primarily identified as excellent producers of lipase in modified lipase assay media. Further it was found out that among five, two isolates of fungi were producing lipase extracellulary and their activity was tested using titrimetric assays. The two fungi Aspergillus fumigatus and Sporotrichum thermophile were found to be the excellent producers of lipase. These isolated fungi have been tested for their pH-activity relationships and pH range is 4-8.5. Excellent lipase specific activity was observed from pH 6-7.

KEYWORDS: Sporotrichum thermophile; Aspergillus fumigatus; Lipase

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