Volume 6, number 1
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Studies on lectins from Abrus precatorious L

A. G. Ingale

Department of Biotechnology, School of Life Sciences North Maharashtra University, Jalgaon India.

ABSTRACT: Lectins are proteins of non-immune origin that recognise carbohydrates in a very specific way and can be used as probes to detect different types of glycoconjugate molecules in cells. The lectin is a metalloprotein that strongly agglutinates rabbit erythrocytes and is not inhibited by various simple sugars. Lectins are a heterogeneous class of proteins (or glycoproteins) that recognize carbohydrate moieties of glycoconjugates with high specificity and bind to them in a reversible way without altering their covalent structures. The purified lectin contains carbohydrate. A lectin from Abrus precatorious seeds was extracted by treating seed meal (50g) with Tris HCl and purified by affinity chromatography on Sephadex G-50 column. Subunit molecular mass was determined and fragments were estimated by SDS-PAGE. Haemagglutination assays were carried out in microtitration plates. Only one protein band with an apparent molecular mass of 32kDa was found.

KEYWORDS: Abrus precatorious; lectin; Seed protein

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Ingale A. G. Studies on lectins from Abrus precatorious L. Biosci Biotechnol Res Asia 2009;6(1)
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Ingale A. G. Studies on lectins from Abrus precatorious L. Biosci Biotechnol Res Asia 2009;6(1) . Available from: https://www.biotech-asia.org/?p=8437

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