Studies on lectins from Abrus precatorious L
A. G. Ingale
Department of Biotechnology, School of Life Sciences North Maharashtra University, Jalgaon India.
ABSTRACT: Lectins are proteins of non-immune origin that recognise carbohydrates in a very specific way and can be used as probes to detect different types of glycoconjugate molecules in cells. The lectin is a metalloprotein that strongly agglutinates rabbit erythrocytes and is not inhibited by various simple sugars. Lectins are a heterogeneous class of proteins (or glycoproteins) that recognize carbohydrate moieties of glycoconjugates with high specificity and bind to them in a reversible way without altering their covalent structures. The purified lectin contains carbohydrate. A lectin from Abrus precatorious seeds was extracted by treating seed meal (50g) with Tris HCl and purified by affinity chromatography on Sephadex G-50 column. Subunit molecular mass was determined and fragments were estimated by SDS-PAGE. Haemagglutination assays were carried out in microtitration plates. Only one protein band with an apparent molecular mass of 32kDa was found.
KEYWORDS: Abrus precatorious; lectin; Seed protein
Download this article as:![icon_pdf](https://agriculturejournal.org/wp-content/uploads/2014/03/icon_pdf.png)
Copy the following to cite this article: Ingale A. G. Studies on lectins from Abrus precatorious L. Biosci Biotechnol Res Asia 2009;6(1) |
Copy the following to cite this URL: Ingale A. G. Studies on lectins from Abrus precatorious L. Biosci Biotechnol Res Asia 2009;6(1) . Available from: https://www.biotech-asia.org/?p=8437 |