Study on the Extraction and Charecterization of Outer Membrane Proteins of E. Coli From Poultry

M. Suresh¹, Y. Hari Babu¹, S. Venkateshwar² and M. Narsi Reddy²*

¹Department of Veterinary Microbiology, Veterinary College, Bidar - 585 401 India.

²Centre for Biotechnology, University College of Technology, Osmania University, Hyderabad - 500 007 India.

Corresponding Author E_mail: mnreddydr@gmail.com

ABSTRACT: The outer membrane proteins of avian E. coli play significant role in the pathogenesis of avian colibacillosis and have been identified as potent immunogen. Outer membrane proteins may be pivotal to the virulence of the organism and given their prominence on the cell surface may prove to be suitable candidate for vaccine development.The E. coli cultures grown under the above conditions were pelleted at 4000 rpm for 10 minute at 4°C. The pellet was washed three times in 10mM HEPES buffer and finally re-suspended in 10 mM HEPES buffer. Outer membrane proteins were extracted as per the method described by Bolin and Jensen (1987).The protein profiles of outer membrane proteins were analyzed by SDS-PAGE in vertical slab gel unit.Analysis of the OMP profiles of E.coli serotype O6 revealed three bands approximately of 34, 38 and 44 kDa molecular weight. Serotype O20 revealed the presence of two thick bands of 34 and 38 kDa molecular weight and faint bands of 21 kDa and 58 kDa molecular weight. Similarly O60 serotype shows the presence of two major OMP bands of approximately 38, 43 kDa molecular weight. The serotype O109 had two thick bands of 34 and 38 kDa molecular weight and 21 kDa, 29 kDa and 58 kDa molecular weight faint OMP bands.

KEYWORDS: OMP; E.coli; colibacillosis; SDS-PAGE

[ HTML Full Text]