Volume 5, number 2
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Restriction digestion of EcoRI in E. coli RY13 plasmid DNA

C.S. Senthilkumar, Tahir M. Malla, Sameena Akhter, Aadil Beg And N. Ganesh

Department of Research, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal India.

ABSTRACT: An attempt was made to support the evidenced research of restriction enzyme EcoRI digests plasmid DNA of a significant genetic strain E.coli RY13 by targeting their specific sequence GAATTC. E.coli RY13 was harvested with special antibiotics in terrific broth and plasmid DNA was isolated and digested with EcoRI, then electrophoresed in 0.8% Agarose gel. EcoRI cleaved plasmid DNA into fragments similarly in size that confirmed the presence of target sequence in the E. coli RY13 genome.

KEYWORDS:

Restriction digestion; plasmid DNA; EcoRI, agarose gel; electrophoresis.

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Senthilkumar C.S ,Malla T.M , Akhter S, Beg A ,Ganesh A. Restriction digestion of EcoRI in E. coli RY13 plasmid DNA.Biosci Biotechnol Res Asia 2008;5(2).
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Senthilkumar C.S ,Malla T.M , Akhter S, Beg A ,Ganesh A. Restriction digestion of EcoRI in E. coli RY13 plasmid DNA.Biosci Biotechnol Res Asia 2008;5(2).Available from:

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