Effect of Culture Conditions on Growth And Extracellular Lipase Production by Sporosarcina Sp. Isolated From Oil Extraction Mill Soil

Rakesh Kumar¹*, Pratibha Bhargav², Arun Kumar³ and Deepak Singh¹

¹Department of Zoology & Biotechnology, Hemwati Nandan Bahuguna Garhwal University, Srinagar (Garhwal) Uttarakhand (India). ²Department of Biotechnology, Dolphin (PG) College of life Sciences, Chunnikalan, Distt- Fatehgarh Sahib, Punjab (India). _³Department of Biotechnology, Dolphin (PG) Institute of Biomedical & Natural Sciences Dehradun - 248 001 (India).

ABSTRACT: Sporosarcina sp. was isolated from oil extraction mill soil by enrichment culture technique that had the ability to produce lipase enzyme. Culture conditions of Sporosarcina sp. were optimized at shake flask level. Maximum enzyme production was achieved in between 96h and 120h of cultivation. The rice bran oil was found to be the most suitable lipid for enzyme production (8.52U/ml) among coconut oil, olive oil, refined oil and mustard oils. The enzyme had temperature optima at 37°C and its peak activity was at pH 4.0. Among different nitrogen sources, ammonium sulphate was found to be the most suitable nitrogen source at which it showed the highest activity (10.41U/ml). Potassium dihydrogen phosphate enhances the enzyme production as an inorganic salt into the medium. Tween 20 was used as biosurfactant which increases the enzyme titer to 1.5 times.

KEYWORDS:

Lipase; Sporosarcina sp; enzyme assay; nutritional parameters

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