Influence of Nitrogen Source on Endoxylanase Production and the Induction of Other Hydrolases From Bacillus Sp.

C. Karthikeyan

Department of Biotechnology, J.J. College of arts and science Pudukkottai 622 422,

Tamil Nadu, India.

Corresponding Author E-mail:  kamuthikarthee@yahoo.co.in.

ABSTRACT: The present study was aimed for the production of endoxylanases from a Bacillus culture using nutritional parameters especially the nitrogen sources. The different concentration of organic nitrogen sources (Yeast extract, Beef extract and Peptone) and inorganic nitrogen source (Ammonium nitrate, Ammonium Sulphate, Ammonium Chloride, Ammonium Phosphate and Urea) were used. The effect of best organic nitrogen source on different hydrolases induction was also studied. The results were indicated that the better endoxylanase expression at pH 7 from bacteria grown at complex organic nitrogen sources (i.e. 0.25 g %, peptone + 0.25g % beef extract level). From the inorganic nitrogen sources, ammonium sulphate 1mM gave better endoxylanase enzyme production after 48 hours. The optimum concentrations of organic nitrogen sources were also selected to the study of induction of hydrolases like cellulases, xylanases and proteases induction in this culture.

KEYWORDS: Endoxylanase; Bacillus sp; organic and inorganic nitrogen; hydrolases

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