Critical Studies on Physico-Chemical Parameters for the Production Of Protease Through SSF With Bacillus Subtilis NCIM 2724 Using Black Gram Husk As Substrate

Haritha Meruvu and Meena Vangalapati*

Center for Biotechnology, Department of Chemical Engineering, College of Engineering, Andhra University, Visakhapatnam - 530 003 India.

ABSTRACT: Protease enzyme is commercially produced by the cultivation of Bacillus subtilis through solid state fermentation using black gram husk which an agricultural waste of low economic value. The production rate is augmented by using carbon and nitrogen supplements like maltose and ammonium chloride. The reaction was carried out by optimizing various physico chemical parameters. The present study reveals that maximum protease enzyme was produced at incubation time 24 hrs, temperature 27oC, moisture content 40% w/v, inoculum level of 2 % w/v, and with substrate concentration of 10 g, pH 7.0, maltose concentration 1.5 % w/w and ammonium chloride concentration 2% w/w with a yield of 693.4 U/ml.

KEYWORDS:

Protease; SSF(solid state fermentation); Bacillus subtilis; physico chemical parameters

[ HTML Full Text]