Compatibility of Cardiac Muscle Cells on Coated-Gelatin Electro-Spun Polyhydroxybutyrate/valerate Nano Fibrous Film

M.Montazeri¹, N.Rashidi², E.Biazar^3,4,*, H.Rad Gh⁵, M.Sahebalzamani⁶, S.Heidari K.³ and A.Majdi⁷

¹Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran Heart Center Hospital, Tehran (Iran). ²Faculty of Medical Sciences, Tehran University of Medical Sciences, Shariati Hospital, Tehran (Iran). ³Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ⁴Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran. ⁵Faculty of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon (Iran). ⁶Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran (Iran). ⁷Young Researchers Club, Tonekabon Branch, Islamic Azad University, Tonekabon (Iran).

ABSTRACT: Tissue engineering is defined as the designing and engineering of structures to rebuild and repair a body damaged tissue. Scaffolding Poly Hydroxy Butyrate Valrate (PHBV) has shown good biocompatibility and biodegradable properties. Nanofibers have improved the performance of biomaterials, and could be considered effective. One of the important methods for designing nanofiber scaffold is the electrospinnig method. In this study, PHBV nanofibers were well designed; then, modified with the immobilized gelatin via the chemically method. The samples were evaluated by ATR-FTIR, SEM, and finally, myocardiocyte culture. ATR-FTIR structural analysis showed the presence of gelatin on the nanofiber surfaces. The SEM images showed the size average of nanofibers as to be about 280 nm; that increased with a gelatin coating up to 500 nm. Cellular investigations (myocardiocyte) showed better adhesion and cell growth and proliferation of coated samples than uncoated samples. In this work, the PHBV nanofibers with a size average about 280 nm were designed. Nanofibers were successfully coated with gelatin via the chemically methods. These gelatin-coated nanofibers could be used well for heart tissue engineering.

KEYWORDS: PHBV; Nanofiber; Gelatin Coating; Cellular Investigation; Cardiac Muscle Cells

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