In vitro Antibacterial Activity of Organic Solvent Seed Coat Extracts of Borassus flabellifer Linn

G. R. Duddukuri*, Y. N. Sastry, D. S. V. G. K. Kaladhar, K. Kamalakara Rao and K. Krishna Chaitanya

Department of Biochemistry and Bioinformatics, GITAM University, Visakhapatnam - 530 045 India.   Corresponding Auhtor E-Mail: dgrao1@gmail.com

ABSTRACT: The in vitro antibacterial activity of organic solvent extracts of Borassus flabellifer L. (Arecaceae) seed coat (soft outer shell) was studied by agar well diffusion method. The tender seed coat of the plant Borassus flabellifer was extracted with different organic solvents viz. petroleum ether, acetone, ethyl acetate, ethanol and methanol. The effect of antibacterial potential was examined against Gram positive bacteria i.e., Staphylococcus aureus, Bacillus subtilis and Gram negative bacteria i.e., Escherichia coli, Klebsiella pneumoniae. Among the different organic solvent extracts of the seed coat tested, methanol extract has showed consistently significant inhibitory activity on different bacterial species. Furthermore, the minimum inhibitory concentration studies carried out by broth dilution assay and found the MIC ranged between 3.12 to 6.25 mg/ml.

KEYWORDS: Borassus flabellifer; Seed coat; Antibacterial assay; MIC

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