A Study on Poly Hydroxy Butyrate (PHB) Production and Its Application from Pseudomonas Spp. (MTCC)

S. Sujitha¹ and D. Ravi²

¹CMS College of Science and Commerce, Coimbatore - 641 006 India.

²Government Arts College, Higher Education Department, Coimbatore - 641 018 India.

ABSTRACT: By considering the industrial interest on poly hydroxyl butyrate and its high production cost current work has been undertaken for the production of PHB from five different Pseudomonas species. By using different types of substrate waste, like Food Industry wastes(Bagasse,Wheat bran, Whey), Chemical Industrial waste (Activated sludge) and Wood industry waste (saw dust ) were used as a cheap substrate to minimize the production cost of PHB.Accumulate through metabolic mechanism microbes accumulate secondary metabolites such as PHB in the form of granules in the intracellular region. This was analyzed and identified by Nile blue A and Sudan black method.PHB production in various industrial waste based medium was studied by Crotonic acid method. The pure form of PHB was collected and biochemically analyzed by TLC method .The biodegradation of PHB was studied by using Fungal isolates through PHB incorporated agar medium assay. The clear zone around the colonies was measured to evaluate the activity of the isolates. The fungal isolates were found to degrade these polymers more rapidly within 3 to 7 days due to their versatile depolymerase activities. These PHB has another role in biochemical mechanisms.ie, poly 3 hydroxy butyrate act against the pathogens in the fishes and other aquaculture spp.(specifically in nauplii-var.).

KEYWORDS: Poly- b- hydroxy butyrate; PHB; Bioplastic; Biodegradation fungi; PHB application.

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