In Vitro Anti Inflammatory Activity of Methanolic Extract of Bacopa Monniera by HRBC Membrane Stabilisation

Sharan Suresh Volluri¹, Srinivasa Rao Bammidi², Seema Chaitanya Chippada² and Meena Vangalapati²

¹Department of Biotechnology, DVR and HS MIC College of Technology, Kanchikacherla India.

²Center for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam - 530 003 India.

ABSTRACT: Bacopa monniera also referred to as Bacopa monnieri, Herpestis monniera, water hyssop, and “Brahmi,” has been used in the Ayurvedic system of medicine for centuries. Phytochemical analysis of B. monniera plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides,bacosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti inflammatory activity, so our present work aims at evaluating the in vitro anti inflammatory activity of B. monniera by HRBC membrane stabilization. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti inflammatory activity. The percentage of membrane stabilisation for methanolic extracts and Diclofenac sodium were done at different concentrations. The maximum percentage of membrane stabilization and hemolysis of B. monniera extracts were found to be 92.92±1.41% and 7.08±1.17% at a dose of 2000 ?g/ml respectively, when compared to standard Diclofenac sodium was found out to be 98.76±1.26% and 1.24±0.87% at a dose of 2000 μg/ml respectively .Therefore, our studies support the isolation and the use of active constituents from B. monniera in treating inflammations.

KEYWORDS: Anti-inflammatory; Bacopa monniera; Diclofenac sodium; Human Red Blood Cell (HRBC)

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