Bacteriocin Producing Bacillus thuringiensis and its Effect on Human Pathogens
D. Kurup, H. M. Lele and B. M. Nabar*
Postgraduate Department of Microbiology, Smt. C H M College (Affiliated to University of Mumbai), Ulhasnagar - 3 India.
Corresponding Author E-mail: belamsn23@gmail.com
ABSTRACT: Sixty five Bacillus thuringiensis strains were isolated from soils of the western ghats of Maharashtra. From sixty five isolates of B. thuringiensis; ten B. thuringiensis strains were screened for their cry gene profile (cry2, cry3 and cry6) and capacity to express bacteriocin-like agents. B. thuringiensis isolate no.9, showed antagonistic activity towards Corynebacterium diphtheriae, Rothia dentocariosa, Moraxella catarrhalis, Neisseria polysaccharea, Staphylococcus aureus, Streptococcus pyogenes, and other Bacillus sp.; The partially purified bacteriocin (PPB) obtained from isolate no.9 by salt precipitation was studied by SDS-PAGE had an apparent Molecular weight of 43kDa. Effect of temperature and pH on activity of PPB was studied. This novel identified and characterized partially purified bacteriocin can be effective in control of human pathogen such as Coryne. diphtheriae it may play an interesting role in therapeutic science.
KEYWORDS: Bacillus thuringiensis; Bacteriocin; SDS PAGE; Corynebacterium diphtheriae
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Copy the following to cite this article: Kurup D, Lele H. M, Nabar B. M. Bacteriocin Producing Bacillus thuringiensis and its Effect on Human Pathogens. Biosci Biotech Res Asia 2011;8(2) |
Copy the following to cite this URL: Kurup D, Lele H. M, Nabar B. M. Bacteriocin Producing Bacillus thuringiensis and its Effect on Human Pathogens. Biosci Biotech Res Asia 2011;8(2). Available from: https://www.biotech-asia.org/?p=9500/ |