Extraction of Carbohydrate from Sweet Orange Peels  (Citrus sinensis L.) and their Identification via LC /MS and Thin Layer Chromatographic Analysis

C. S. Chidan Kumar¹, R. Mythily and S. Chandraju*

¹Department of Studies in Sugar Technology, Sir M. Vishweshwaraya Post-Graduate Centre,University of Mysore, Tubinakere, Mandya - 571 402 India.

²Department of Chemistry, G. Madegowda Institute of Technology, Bharathi Nagar - 571 422 India.

Corresponding Author: Email- chandraju1@yahoo.com

ABSTRACT: A rapid, sensitive extraction method was developed using the mixture Methanol –Dichloromethane - Water (MDW) (0.3:4:1, v) and MeOH-H2O phase was assayed for sugar analysis. Photodiode-array detection (DAD) has been used to prove the extracted compound is UV inactive, High-performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) coupled to electrospray ionization mass spectrometric (ESI-MS) detection in the positive ion mode gave MS and MSn fragmentation data which were employed for their structural characterization. The various standard sugars were spotted using the solvent system n-butanol-acetone-pyridine-water (10:10:5:5, v) in the cellulose layer for TLC analysis which indicated the presence of fructose, galactose, glucose, arabinose and Xylose. This is the first assay of the sugar profile of the orange peels, which can be further developed for characterization and evaluation of their quality with regards to their sugar composition.

KEYWORDS: Sugar extraction; Orange peels; UV inactive; Separation; LC/MS; TLC

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