Bacteriological and Toxicological Qualities of Dialysis Water and Associated Pyrogenic Reactions Involved in Haemodialysis Patients

Samy A. Selim¹, Sahar M. El Alfy¹, Atef M. Diab², Mohamed², H. Abdel Aziz³and Mona S. Ewada³

¹Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Al-Jouf University, Saudi Arabia. ²Department of Botany, Microbiology Section, Faculty of Science, Suez Canal University, Ismailia- 415 22, Egypt. ³Health Insurance Authority Hospital, Suez Governorate, Egypt.

ABSTRACT: The present work surveyed Haemodialysis (HD) units, in order to assess the bacteriological and toxicological qualities of dialysis water and dialysate. Also determine experimentally to how extent the bacterial activities are involved in immune depression for the patients. The most commonly isolated Gram-negative bacteria from treated water were Aeromonas spp. (22.2%). Endotoxin concentrations ranged between 3-113 (EU/mL) for water and dialysate respectively. The haematological analysis had non significant except haemoglobin level had a significant values (p = 0.02). The clinical data showed comparable difference than control patients. Missing, out of work, bypassed and even erroneously arranged treatment facilities are some of the reasons responsible for the detected high levels of bacterial and endotoxin in final product water. The presence and growth of bacteria in water and dialysate should be controlled and the endotoxin testing must be a part of the regular quality control regime, to minimize the risk of adverse reactions in HD patients.

KEYWORDS: Bacteria; endotoxins; haemodialysis patients; immune depression; Haemodialysis (HD)

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