Anti-Osteoporotic Activity of Ostinu, A Herbo-Mineral Preparation in Ovariectomized Rats

Shahabuddin Bahmani¹*, H. Ramesh², M. Majid Iqbal² and K. S. Rao²

¹Department of Pharmacology, RKK Samithi’s, College of Pharmacy, Naubad, Bidar - 585 402 India.

²Department of Pharmaceutical Chemistry and Department of Pharmaceutics, RKK Samithi’s, College of Pharmacy, Bidar - 585 402 India.

Correspondong Author e-mail:bahmani@rediffmail.com

ABSTRACT: Ostinu is a herbomineral formulation consisting of Terminalia arjuna, Phyllanthus embelica, Cissus quandrangularis, Commiphora mukul, Withania somnifera, Shanka bhasma, Pravala bhasma, Godanti bhasma and Kapardika bhasma. Aim of this study was to evaluate effect of Ostinu on bone loss induced by ovariectomy in wistar rats. Female Wistar rats weighing 170-200gm were divided into five groups of eight animals in each group. Normal and control groups we adminstered vehicle. Standard group received Alendronate (ALN) sodium 1mg/kg.b.w. Test groups were treated with Ostinu 80mg/kg (Ostinu-1) and 160mg/kg (Ostinu-2). All drugs were administered orally daily. To perform ovariectomy animal was anesthetized with ketamine (60mg/kg) and diazepam (5mg/kg). Bilaterally ovariectomy was performed in all groups except normal group, which were sham operated. After 12 week of treatment all the animals were sacrificed with overdose of ether anesthesia. Blood was collected from sacrificed animals and serum calcium and phosphorus was estimated. Femur and tibia were collected and evaluated to obtain weights, ash weight, ash calcium & phosphorus content and femur volume & density. Scanning electron microscopy on femur is carried out to determine bone resorption. Ostinu 160mg/kg and Alendronate very significantly (p< 0.01) prevented decrease in femur volume & density, femur ash calcium & phosphorus and significantly (p< 0.05) prevented decrease in tibia weight, serum calcium and phosphorus compared to control. Alendronate has very significant (p< 0.01) effect on femur weight and femur ash weight but Ostinu 80mg/kg has only significant ((p< 0.05) effect compared to control. The results show that Ostinu prevented bone loss in post-menopausal osteoporosis in Wistar rats.

KEYWORDS: Ostinu; Osteoporosis; Ovariectomy; Herbal formulation.

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