Helicobacter Pylori and the Herbal Compound Effect

N. Rahimifard^1,2,*, M. Rabiei^1,2, L. Beitolahi³ and K. Ahi¹

¹Departments of Biology and Microbiology, Food and Drug Laboratory Research Center (FDLRC), Tehran, Iran. ²Departments of Biology and Microbiology, Food and Drug Control Laboratories (FDCLs), Ministry of Health (MOH), Tehran, Iran. ³Department of Microbiology, Kashan University of Medical Sciences, Tehran, Iran.

ABSTRACT: Helicobacter pylori, a gram negative microaerophilic bacterium, infection is now recognised as a worldwide problem. The growing problem of antibiotic resistance by the organism demands the search for novel herbal compounds. The present study is designed for evaluating the antimicrobial effect of methanol extract of this remedy on clinical isolates of H.pylori in Kashan to identify potential sources of cheap stating materials for the synthesis of new and more effective drugs. This study was taken from 20 Helicobacter pylori samples isolated from patients with gastrointestinal disorders. The samples were cultured on Columbia agar base plates (Merck) with supplements. Plates were incubated at 37°C for 3-5 days in a microaerophilic environment (anaerocult C, Merck). The isolates that grown on plates were identified by bacteriological tests. The micro dilution broth method was used to determine the susceptibility of methanol extract of ginger on 20 isolates of H.pylori with a serial dilution of 1000, 500, 250, 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78 µg/ml. The MIC of extract ranged from 3.125-25 µg/ml for H.pylori, the MIC of 12 (60%) isolates were 3.125µg/ml ,4 isolates(20%)were 12.5µg/ml, 3 isolates(15%)were 6.25µg/ml, 1 isolate (5%)was 25µg/ml. The methanolic extract of ginger may contain compounds with therapeutic activity, and the most effective MIC (60%) against H.pylori were 3.125µg/ml. We also understand that H.pylori has high sensivity against ginger.

KEYWORDS: H.pylori; methanolic extract of ginger; MIC; gasterointestinal disorder

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