Volume 9, number 1
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Papain Partial Purification Improves its Sensitivity towards Heavy Metals Papain Partial Purification

N. A. Masdor* and N. A. M. Said

Biotechnology Research Centre, MARDI, P. O. Box 12301, 50774 Kuala Lumpur, Malaysia.

Corresponding Author E-mail: azlina@mardi.gov.my

ABSTRACT: The protease papain, in combination with the dye-binding coomassie protein assay of its substrate has been shown to be sensitive towards toxic heavy metals and has good potential as a preliminary screening method for detecting heavy metals in various samples. The original assay uses crude preparation and there is a possibility to improve its sensitivity towards heavy metals by removing interfering proteins. Using a combination of ion exchange and gel filtration chromatography, a 3.3 fold purification was achieved, and the partially purified fraction showed significantly lower IC50s (p<0.05) for all heavy metals tested except for zinc, than the crude fraction. This demonstrates that partial purification improves the sensitivity of the papain inhibitive assay towards heavy metals.

KEYWORDS: Papain; Partial purification; Heavy metals

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Masdor N. A, Said N. A. M. Papain Partial Purification Improves its Sensitivity towards Heavy Metals Papain Partial Purification. Biosci Biotech Res Asia 2012;9(1)
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Masdor N. A, Said N. A. M. Papain Partial Purification Improves its Sensitivity towards Heavy Metals Papain Partial Purification. Biosci Biotech Res Asia 2012;9(1). Available from: https://www.biotech-asia.org/?p=9595

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