Volume 8, number 2
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RAPD Analysis of Leaf Litter Associated Bacterial Population From Canal, Kollemcode

P. A. Mary Helen*, Deepi, S. Jaya Sree, A. M. Nizzy and S. M. Madoen Abisha

Department of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakkavilai - 629 153 India.

Corresponding Author E-mail: helen_CD@yahoo.co.in

ABSTRACT: Random amplified polymorphic DNA (RAPD) analysis was performed on 7 bacterial isolates isolated from leaf litter from canal Kollemcode. The isolates produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. Five primers such as OPA-06, OPA-07, OPA-17, OPAD-02, OPAD-07 were used for the RAPD analysis. DNA banding patterns generated by RAPD were scored for the presence (1) or for absence (0) of each amplified band. For genetic distance analysis, using NTSYS software Cluster analysis was based on similarity matrices using the unweighted pair group method analysis (UPGMA) program in the software package. The Jaccard coefficient was used for dendrogram construction. A dendrogram based on these results showed a high level of genetic similarity between different bacterial isolates, and genetic differences were expressed in clusters. The genetic difference between the overall population was low and showed similarity with each population.

KEYWORDS: RAPD analysis; Dendrogram; Cluster analysis; Genetic distance; Similarity matrix

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Helen P. A. M, Deepi, Sree S. J, Nizzy A. M, Abisha S. M. M. RAPD Analysis of Leaf Litter Associated Bacterial Population From Canal, Kollemcode. Biosci Biotech Res Asia 2011;8(2)
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Helen P. A. M, Deepi, Sree S. J, Nizzy A. M, Abisha S. M. M. RAPD Analysis of Leaf Litter Associated Bacterial Population From Canal, Kollemcode. Biosci Biotech Res Asia 2011;8(2). Available from: https://www.biotech-asia.org/?p=9660

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