Instantaneous Biosynthesis of Noble Metal (Ag) Nanoparticles Assisted by Edible Mushroom Biomass and Their Treatment of Textile Fabrics

Eman M. A. El-Taher

The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt.

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1027

ABSTRACT:

Many useful properties of locally edible mushrooms are still being studied. This study shows for the first time, the green biosynthesis of silver nanoparticles (AgNPs), with the helping of the mycelial mat of local-edible mushroom; Pleurotus ostreatus. The present process is an excellent candidate for industrial scale production of nanoparticles. Hence, two modes of biosynthesis were identified: with the mycelia, extracellular and intracellular biosynthesis. The ability of P. ostreatus to produce AgNPs under sun light, instantaneously, is reported. Optical detection followed by confirmation through spectroscopic analysis suggests that this fungus can be used for the purpose of safe and sure synthesis of AgNPs. For morphological details of cell structure, transmission electron microscopic (TEM) analysis was carried out. TEM was also showed the nanoparticles, were mostly spherical, polydispersity and distribution of AgNPs in the range of 3 - 25 nm with an average size of 10 nm. EDX analysis recorded that, signal from the Ag atoms in the nanoparticles is observed, while, signals from C, O, Na, Si, P, S, Cl, K and Ca atoms were also recorded. The current research confirmed that, these nanoparticles had potent antimicrobial effect on pathogenic bacteria Bacillus subtilis and Escherichia coli and the pathogenic fungus Aspergillus niger. Moreover, focus has been given to the development of an efficient and eco-friendly viable process for sterilization of textile fabrics, which are using at ancient pharaonic works.

KEYWORDS: Mushrooms mycelia; Silver nanoparticles; Green synthesis; Antimicrobial activity

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