Relation between Homocysteine and Vitamin B6 on Biochemical Bone Turnover Markers in Ovariectomized Rats

Noorah Saleh Al-Sowayan

Department of Biology, Faculty of Science, Qassim University, P. O. Box: (30230), Buraydah (51744), Saudi Arabia.   Corresponding Author E-mail: knaaj1@yahoo.com

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1031

ABSTRACT:

The purpose of this study was to examine the associations of serum concentrations of vitamin B6 and homocysteine with bone loss and fracture risk in postmenopausal female rats .Thus we try to visualize the effect ofovariectomy , estrogen replacement therapy and vitamin B6 supplementation on homocysteine levels and parameters of bone biomarkers. Adult female rats were classified as healthy control and ovariectomized control rats, the third group were ovariectomized rats received estrogen daily, the fifth and sixth group were ovariectomized rats received normal diet fortified with vitamin B6, and ovariectomized rats received estrogen daily with diet fortified with vitamin B6 respectivly. Fasting blood samples were measured for homocysteine, calcium ion, osteocalcin hormone, vitamin D3concentrations and alkaline phosphatase activity. The data from this study showed that indices of both bone resorption and formation increased markedly after ovariectomy.Serum homocysteine (Hcy) level ,vitamin. D3 and alkaline phosphatase activity were higher with insignificant changes in serum calcium and osteocalcin level in ovariectomized controlcompared with control healthy rats . Oral estrogen administration to ovariectomized rats induced higher concentration of serum Hcy when compared to normal control rats but Hcy concentration, alkaline phosphatase were decreased with insignificant decrease in serum Ca+2, vitamin D3 and increased osteocalcin level compared with ovatiectomized control group .Supplementation of vitamin.B6 alone to ovariectomized rats induced significant increase in osteocalcin accompanied with insignificant changes in serum Ca+2, and alkaline phosphates . VitaminD3 significantly increased in vitaminB6 treated rats than ovariectomized control rats.Combined treatment of ovariectomized rats with estrogen andvitamin B6 induced insignificant decrease in serum C+2 and return its level to normal value ,accompanied with significant increase in vitamin.D3 and osteaocalcin with decreased activity of alkaline phosphatase compared with withovariectomized group. The prevention of osteoporosis by identifying risk indicators as well as the development of new treatment strategies are major issues . Homocysteine is not only a risk factor ,but also a player in bone metabolism . However , this trial suggests that Hcy –lowering therapy may prevent bone loss in postmenopausal women .

KEYWORDS: Menopause; Estrogen deficiency; Bone disease, Rats.

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