Volume 9, number 2

Characterization of Immobilized L-Asparaginase Produced from Pseudomonas aeruginosa 50071 by Solid State Fermentation

Mohsen M. S. Asker1, Sahera F. Mohamed1.2* and Osama H. El-sayed2

1Microbial Biotechnology Department, National Research Center, Dokki, Cairo, Egypt. 2Chemistry Department, College of Science, Jazan University, Jazan, KSA.

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1034

ABSTRACT: The natural polymer sodium alginate is a macropolysaccharide Produced from brown algae has many applications. Anti-leukemia enzyme L-asparaginase (L-ASNase) produced from Pseudomonas aeruginosa 50071 by solid state fermentation was covalently conjugated on sodium alginate. SDS-PAGE showed that the purified L-ASNase was homogeneous with molecular weight of ~ 113 KDa. The immobilized enzyme on 3% sodium alginate showed specific activity of 62 U/mg protein. The immobilized L-ASNase maintained over 90 % of the original activity of free enzyme. Also it showed a slightly thermal stability where the activation energy of denaturation were 28 and 35 Kcal mol-1 for both native and immobilized enzyme, respectively. The Km of sodium alginate immobilized enzyme was about 2 times lower than that of the free enzyme.

KEYWORDS:

Immobilization; L-asparaginase; pseudomonas aeruginosa; solid state fermentation.

Copy the following to cite this article:

Asker M. M. S, Mohamed S. F, El-sayed O. H. Characterization of Immobilized L-Asparaginase Produced from Pseudomonas aeruginosa 50071 by Solid State Fermentation. Biosci Biotech Res Asia 2012;9(2)
Copy the following to cite this URL:

Asker M. M. S, Mohamed S. F, El-sayed O. H. Characterization of Immobilized L-Asparaginase Produced from Pseudomonas aeruginosa 50071 by Solid State Fermentation. Biosci Biotech Res Asia 2012;9(2). Available from: https://www.biotech-asia.org/?p=9932

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