Assessing Genetic Variation of Dog Rose (Rosa Canina L.) in Caspian Climate

Aptin Rahnavard^1*, Ghavamaldin Asadian¹, Khalil Pourshamsian² and Mariamalsadat Taghavi³

1Department of Medicinal Plants, Islamic Azad University, Tonekabon Branch, Iran. 2Department of Chemistry, Islamic Azad University, Tonekabon Branch, Iran. 3Agricultural Engineering Organization of Tonekabon, Iran.

DOI : http://dx.doi.org/10.13005/bbra/1102

ABSTRACT: Dog rose is one of the important rose species in Iran that the distant past had been considered due to nutritional value and medicinal. Despite its long history of use, due to poor information on the genetic modification of plants has been done resources inheritance. In this study was to assess the genetic diversity. Total of 30 genotypes Dog rose from areas of northern Iran in the Caspian region (provinces of Guilan and Mazandaran) were evaluated using 25 RAPD primers. The number of bands produced total of 202 and for each primer were measured in a bands with an average 8-band .The number of polymorphic bands per primer ranged from 1 to 13 and the bands were in the range of 300 to 3000 bp. Based on the results OPA-04 primer with 13 bands and PRA-1, E-09 and A-04 with 5-band were created maximum and minimum number of amplified fragments. Molecular marker genotypes showed a high degree of polymorphism. Studied genotypes based on RAPD results were divided into 2 groups and 2 subgroups. Most similar in subgroups A2 and B group was the lowest.

KEYWORDS: Rosa canina spp.; RAPD marker; Genetic Variation; Caspian climate

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