Characterization and Antibiogram Study of E.coli Clinical Isolates

G.A. Kamble¹, R.C. Shah² and P.M. Tumane³

1Pulses Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola - 444 104, India. 2Department of Microbiology, J.M. Patel College, Bhandara - 441 904, India. 3P. G. Department of Microbiology, RTM Nagpur University, Nagpur - 440 033, India.

DOI : http://dx.doi.org/10.13005/bbra/1130

ABSTRACT: Hospital environment is most suitable reservoir for nosocomial pathogens. Indiscriminate use of antibiotics has led to the global drug resistance in these microbes leading to several health hazards. E.coli is one such bacterial pathogen. It is therefore important to isolate and identify it from various clinical samples and study its antibiogram. In present study a total of one hundred samples of blood, urine, pus and stool collected from various pathology laboratories were used for isolation of E.coli. Twenty eight samples were reported positive for the occurrence of E.coli in them. The highest rate of incidence was found in stool samples whereas all the pus samples analyzed were negative. Antibiogram study of these E.coli clinical isolates showed resistance to ampicillin and cephalothin followed by amoxycillin and ciprofloxacin. Absolute sensitivity to chloramphenicol and tetracycline followed by norfloxacin, imipenem, streptomycin, moxifloxacin and ofloxacin was observed. All most all isolates showed multiple drug resistance.

KEYWORDS: Antibiogram; E.coli; Nosocomial infections

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