Comparative Biochemical Study of Aloe-emodin and Barbaloin by Antioxidative and Antiglycation Evaluations in Mice Liver Tissue

Somayeh Amiri¹*, Masoud Mashhadi Akbar Boojar², Ramazan Ali Khavari-Nejad¹ and Shima Aslian¹

¹Department of Biology, Science and Research Branch, Islamic Azad university, Tehran, Iran. ²Faculty of Biological science, Kharazmi University, Tehran, Iran.

ABSTRACT: Aloe vera is widely used for medicine, dietary supplements, and cosmetic purposes. In recent years, the consumption of this plant¼s juice and gel has promoted and with entering them to the body different systems are encountering their effects. Liver as an important organ may response to the main active components of this plant, Aloe-emodin and Barbaloin. The aim of the present study was to investigate the antioxidant and antiglycation properties of these compounds on mice liver tissue at concentrations ranging from 0 to 12 mM. In this study various concentrations (0-12 mM) of Aloe-emodin and Barbaloin were added to liver tissue cultures and after 24h incubation the tests were performed. Enzymatic activities, Malondialdehyde level and DPPH radicals scavenging activity were evaluated by spectrophotometric method. Dityrosine level was measured by HPLC method. Antiglycation properties were evaluated by pentosidine and glyoxal tests. in vitro studies indicated that Aloe-emodin and Barbaloin scavenged free radicals in a dose dependent manner. Also, the results revealed that Aloe-emodin and Barbaloin could increase liver antioxidant enzymes activity including superoxide dismutase, catalase, and glutathione peroxidase at low (0.75-1.5 mM) and high (3-12 mM) concentrations respectively. In other words, the levels of malondialdehyde and dityrosine, as oxidative damage biomarkers, significantly decreased at these concentrations, compared with the control value. Moreover at these doses, these two compounds had significant antiglycation property on the liver tissue as they evaluated by pentosidine and glyoxal tests. Therefore, as these compounds had significant antioxidant and antiglycation properties on the liver tissue, it can be expected that usage of them can be protected the liver tissue against chronic liver disease that is resulting from diabetes, non-alcoholic steatohepatitis (NASH).

KEYWORDS: Aloe-emodin; Barbaloin; Antioxidant enzymes; Oxidative damage biomarkers; Antiglycation

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