PCR Amplification of Chicken Anaemia Virus VP2 Gene in Chicken and Pigeon, in Iran

M. Gholami-Ahangaran¹*, E. Fathi-Hafshejani¹ and R. Seyed-Hosseini²

¹Poultry Diseases Department, Veterinary Medicine Faculty, Shahrekord Branch, Islamic Azad University, PO Box166, Shahrekord, Iran. ²Graduated from Diploma Degree in Poultry Health and Diseases, Shahrekord University, Shahrekord, Iran.

ABSTRACT: The situation of chicken anaemia virus (CAV) infection in different species of birds, exception of chickens is unclear. For this, in this study 375 thymus samples have been collected from 25 broiler chicken with high mortality in Iran. Furthermore, 200 blood samples were collected from 20 apparently healthy pigeon flocks with different ages. After DNA extraction, PCR was carried out to amplify a fragment of 713 bp from the viral protein 2 (VP2) gene of CAV. Results showed that, 58.4% thymus samples from 25 broiler chicken flocks were positive to CAV. In the pigeon flocks, all pigeons (100%) were negative with respect to detection of VP2 CAV genome in blood samples. This concluded that in reverse of chicken, the healthy pigeon cannot be infected to subclinical CAV, in Iran.

KEYWORDS: Chicken; Pigeon; VP2; PCR; Iran

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