Studying the Effect of Vitamin E and Selenium on Liver Enzymes in Chemotherapy Rat with Cyclophosphamide

Mozhdeh Khanalizadeh and Mahmood Najafian*

Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom Iran

ABSTRACT:

Cyclophosphamide is a drug of alkylating group and used widely as an anticancer drug. Vitamin E and selenium have important role in protecting the body by their antioxidant factor. The aim of this study is investigating the effect of selenium and vitamin E on liver enzymes in cyclophosphamide chemotherapy rats. The experimented animals were a total of 42 were Wistar rats which randomly divided into 7 equal groups. CO group did not receive any drug or solvent. SA Group daily received 0.2 ml of physiological saline as drug solvent intraperitoneally. SN group received 1 mg/kg-B.W of sodium selenite intraperitoneally. VE group received 200 mg/kg-BW of vitamin E gavage feeding. CP group received 5 mg/kg-BW of cyclophosphamide intraperitoneally. CP-SN and CP-VE groups once a day received 5 mg/kg-BW of cyclophosphamide intraperitoneally and respectively 1 mg/kg-B.W of sodium selenite and 200mg/kg-BW of vitamin E gavage feeding for 21 days. Then the rats were bled and serum was separated. Then, activities of ALT, AST, ALP enzymes were evaluated by the kinetics method and photometer. The results showed that the activities of measured enzymes in SA, SN and VE groups have not significant different compared to the CO group but the CP group has increased significant and in groups of CP-VE and CP-SN has a decreased significant compared to the CP group. According to the results from studies of liver enzymes we can conclude that cyclophosphamide with increasing active oxygen types, increased the liver enzymes’ activity in serum. Vitamin E and sodium selenite also have antioxidant factor and somewhat reduces the negative effects of cyclophosphamide. So, the use of vitamin E and sodium selenite is recommended for reducing cyclophosphamide toxicity in cancer patients.

KEYWORDS: vitamin E; sodium selenite; cyclophosphamide; liver enzymes

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