Optimization Studies of Alkaline Cellulase from Bacillus licheniformis SM1 Isolated from Rice Agricultural Soil using Taguchi Methodology

Sheelendra M. Bhatt, Pankaj Lakhwan and Shilpa

School of Biotechnology and Biosciences, Lovely Professional University Punjab - 144411, India.

DOI : http://dx.doi.org/ http://dx.doi.org/10.13005/bbra/1539

ABSTRACT:

The aim of the present work was to screen microbes related to alkaline cellulase production from rhizosphere of rice field soil from Phagwara, Punjab. Out of 9 isolates screened, isolate 6th designated as SM1 was selected for alkaline cellulase study. Results of 16S rRNA revealed this strain as Bacillus licheniformis. Various medium conditions affecting cellulase activity such as pH, C/N ratio, Tween-20 and lactose were optimized using L9 conditions by Taguchi methodology in submerged conditions. Factors having significant impact over alkaline cellulase activity were in order pH > C/N ratio > Tween-20 > Lactose. The most optimum conditions for cellulase production was pH 9; C/N, 1:2; Lactose, 1 % (w/v) and Tween 20, 1 %(v/v) at 50 0C.

KEYWORDS: Optimization; Alkaline Cellulase; activity, CMCase;

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