Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure

T. Arunkumar¹*, D. Alex Anand² and G. Narendrakumar³

¹Department of Bioinformatics, Sathyabama University, Chennai – 600119, India. ²Department of Biomedical Engineering, Sathyabama University, Chennai – 600119, India. ³Department of Biotechnology, Sathyabama University, Chennai – 600119, India.

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1555

ABSTRACT:

Secondary structure and phylogenetic efficacy of the internal transcribed spacer-1 (ITS1) in laccase producing organism were studied among 35 species through molecular phylogenetic analyses. The sequence of laccase gene were taken from the NCBI databases and compared with the identified sequence that was coded for laccase. Similar sequences of laccase synthesizing organisms were downloaded from the database and multiple sequence alignment was performed. The most closely related sequences were taken from each organism and RNA structure was predicted. This structure was used for comparative approach of rRNA sequences to be similar for the aligned sequence. Then the phylogenetic analysis was carried for the above sequences and the results were compared with the RNA structure for comparative analysis. During the evolution, the laccase genes from the prokaryotes were differentiated to the higher organisms was reported.

KEYWORDS: Pseudomonas aeruginosa ADN04; Secondary structure; rRNA, Phylogenetic analysis.

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