Partial characterization of Cellulase Production by Jeotgalibacillus marinus MTCC 6233

Ashish Vyas1*, Chayanika Putatunda1 and Deepak Vyas2

¹Department of Microbiology, Lovely School of Technology and Sciences, Lovely Professional University, Phagwara (Punjab), India. ²Lab of Microbial Technology and Plant Pathology, Department of Botany, Dr HS Gaur University (A Central University), Sagar (MP) 470 003, India.

DOI : http://dx.doi.org/10.13005/bbra/2206

ABSTRACT: Cellulases form a very important group of enzymes which find application in a wide array of processes including production of bioethanol from lignocellulosic materials, biopulping, textile processing, as additives in animal feeds and laundry detergents etc. In the present research investigation, Jeotgalibacillus marinus MTCC 6233 was isolated from decomposing cellulosic waste material. The isolate was further screened and characterized for production of cellulases. The bacterial strain was found to be a potent producer of endoglucanase, exoglucanase as well as β –glucosidase. Highest production of all the three cellulolytic enzymes was observed at 6th day of incubation. Activity of all the three enzymes was found to be optimum at pH 6 and temperature 500 C. Endoglucanase activity was found to be inhibited by 2,4-Di Nitro Phenol (2,4-DNP) and HgCl2. Thus, the isolate Jeotgalibacillus marinus MTCC 6233 can be further assessed for these enzymatic activities and may prove to be a potential candidate for production of various cellulolytic enzymes at commercial scale.

KEYWORDS: Jeotgalibacillus marinus; cellulose; endoglucanase (EC 3.2.1.4); exoglucanase (EC 3.2.1.94); β –glucosidase

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