Prevalence of Reactive Oxygen Metabolite Levels in Plasma, GCF and Saliva in Chronic Periodontitis, Chronic Gingivitis and Healthy Periodontium: A Biochemical study

Uma Sudhakar¹, T.Ramakrishnan², A. Rekha³, H.Tamizhchelvan⁴, V. Shankar Ram⁵, Kamal Kannadasan⁶ and S. Parthiban⁷

¹Professor & Head, Thaimoogambigai Dental College, ²Professor, VP, Meenakshi Ammal Dental College, ³PG Student,Thaimoogambigai Dental College, ⁴Professor, Oral Pathology, Sri Ramachandra Dental College, ⁵Reader, Thaimoogambigai Dental College, ⁶Professor, Oral Surgery ,Thaimoogambigai Dental College, ⁷Senior Lecturer, Thaimoogambigai Dental College,

DOI : http://dx.doi.org/10.13005/bbra/2212

ABSTRACT: Chronic Periodontitis, an inflammatory disease caused by oral bacteria stimulates the host cells,neutrophils which release Reactive Oxygen Species(ROS) as a part of immune response. Excess ROS is one of the pathological features in the periodontal lesion.Recently, Reactive Oxygen Metabolites(ROM) were recognized as a useful measure of blood ROS .The aim of this study is to estimate the prevalence of ROM in Plasma, saliva and Gingival crevicular fluid in Chronic Periodontitis, chronic gingivitis and healthy controls. The study population consisted of 45 subjects belonging to both the sexes were randomly selected . Subjects were divided into three groups , Healthy periodontium ( HP)(Group I) and Chronic Gingivitis (CG)(Group II), Chronic Periodontitis(CP) ( Group III). GCF, saliva and plasma were collected in all the three groups to estimate the Reactive oxygen metabolite levels. ROM levels in plasma concentration were almost the same in all the groups [ pvalue 0.13]. The values obtained in saliva and GCF were significantly higher in Chronic Periodontitis group compared to Chronic Gingivitis and Healthy Periodontium.[ pvalue < 0.001]. The results of our study suggested that a significant oxidative stress may occur in Periodontitis. The findings also suggest that it might play an important role in the pathology of Periodontitis and the associated tissue damage.

KEYWORDS: ROM; plasma; saliva ; GCF: Chronic Periodontitis

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