Determination of Trace Amounts of Risperidone in Human Urine Sample by Hollow Fiber Liquid-Phase Microextraction Combined with High Performance Liquid Chromatography

Gaianeh Charmahali¹, Mahnaz Qomi^*, Shadi Akhavan², Meghedi Chaharmahali³ and Fatemeh Fallah Tafti⁴

¹Department of Medicinal Chemistry (Pharmaceutical sciences research center),Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. 3Digestive disease Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. 4Department of Chemistry, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran

ABSTRACT: Risperidone is an antipsychotic drug mainly used to treat schizophrenia. In this paper, for the first time a three-phase hollow ûber liquid-phase microextraction (HFLPME) method combined with high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was presented to detection and quantification of risperidone in urine samples. Different factors affecting the HF-LPME extraction recovery were studied and optimized. Under optimal extraction condition, risperidone could be determined within the linearity range with a good correlation coefficient (r2 > 0.9980). The limit of detection for risperidone was 0.2 ng mL-1, and the intra- and inter-day relative standard deviations were no more than 1.2% and 3.2%, respectively. This procedure afforded a simple, sensitive and inexpensive method with a high extraction efficiency for determination of risperidone. Finally, it was applied to determination and quantification of risperidone in human urine samples.

KEYWORDS: Risperidone; High performance liquid chromatography; Hollow ûber based liquid phase microextraction; Microextraction

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