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Kamala Boonyodying1, Thawatchai Kitti2, Duangkamol Kunthalert1 and Sutthirat Sitthisak1,3
1Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. 2Faculty of Oriental Medicine, Chiang Rai College, Chiang Rai, Thailand. 3Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand
ABSTRACT: Bioremediation is a biological method for removing heavy metals from the environment. Heavy metal binding proteins have been identified in various organisms and have been used to study bioremediation. CXXC and histidine peptide rich motifs are metal binding domains found in proteins of all living organisms. Genes encoding the metal binding domain CXXC motif from the mcsA gene and histidine peptide rich motifs from the copA gene of S. aureus were used to construct 3 recombinant proteins containing various metal binding domains, C4, C8 and C4His recombinant proteins. C4 and C8 recombinant proteins contain four and eight domains of CXXC motifs, respectively. C4His recombinant protein contains four domains of the CXXC motif fused with a histidinerich metal binding domain. Recombinant proteins were tested for metal binding using IAA chromatography. C4 and C8 recombinant proteins bound Cu2+, Zn2+, Cd2+and Co2+ , whereas, the C4His recombinant protein did not bind to any heavy metal tested. E. coli expressed with C4 or C8 recombinant proteins showed increased resistance to Cu2+ and Cd2+. E. coli expressed with C4His recombinant proteins showed increased resistance to Cd2+. E. coli expressed with C4, C8 or C4His recombinant proteins were tested for intracellular bioaccumulation under various heavy metal conditions. The results showed E. coliexpressed C8 recombinant protein had the highest Cu2+ and Cd2+ intracellular bioaccumulation above control. This study shows that metal binding domain recombinant proteins can effectively bind and accumulate various types of heavy metals and are good potential tool for studying bioremediation.
KEYWORDS: Heavy metal binding proteins, Bioremediation, CXXC motif
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| Copy the following to cite this article: Boonyodying K, Kitti T, Kunthalert D, Sitthisak S. Heavy Metal Binding and Accumulation of Genetically Engineered E. coli Harboring the CXXC Motif and Histidine Rich Motif Fusion Proteins. Biosci Biotech Res Asia 2015;12(1) |
| Copy the following to cite this URL: Boonyodying K, Kitti T, Kunthalert D, Sitthisak S. Heavy Metal Binding and Accumulation of Genetically Engineered E. coli Harboring the CXXC Motif and Histidine Rich Motif Fusion Proteins. Biosci Biotech Res Asia 2015;12(1). Available from: https://www.biotech-asia.org/?p=4301 |
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