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Effect of Testosterone on Memory and BDNF Levels of Hippocampus in Gonadectomized Diabetic Rats
Mahboubeh Ebrahimzadeh1, Parviz Shahabi2*, GisouMohaddes2, Shirin Babri2, Mostafa Mohammadi3, Alireza Moslem and Mohammad Mohammad-zadeh4*
1Tabriz University of Medical Sciences, International Branch Aras, Tabriz- Iran 2Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz-Iran 3Department of Physiology, Tabriz University of Medical Sciences, Tabriz-Iran 4Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar- Iran
DOI : http://dx.doi.org/10.13005/bbra/1921
ABSTRACT: The prevalence of cognitive impairment in diabetic patients is more than in non-diabetic ones. BDNF and sex hormones levels play important roles in learning and memory. In this study, the effect of testosterone on BDNF expression and memory was investigated in 60 male Wistar rats were randomly divided into 6 groups (n=10): control (C), Diabetic (Dia), Diabetic-Sham (Sh), Diabetic-Gonadectomized (Dia-Gdx), Diabetic-Testosterone (Dia-T), and Diabetic-Gonadectomized-Testosterone (Dia-Gdx-T). Diabetes was induced by STZ (60mg/Kg). Testosterone (2mg/kg/day) was administered intraperitoneally for 6 weeks. The BDNF levels in the hippocampus were assessed by ELISA kit. Novel object recognition (NOR) test was performed for the investigation of recognition memory. Induction of diabetes did not significantly change the BDNF levels of hippocampus, where as Gonadectomy or testosterone administration in diabetic animal's significantly reduced BDNF levels. Short-term memory significantly was reduced in Dia-Gdx, Dia-T, and Dia-Gdx-T groups compared to the control group; however, Gonadectomy or testosterone therapy had no significant effects on it in diabetic rats. BDNF levels and short-term memory decreased in diabetic groups that received or were depleted from testosterone. It is possible that decrease of BDNF gene expression in the hippocampus caused a short-term memory disturbance.
KEYWORDS: Learning and memory; Diabetes; BDNF; Testosterone
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Copy the following to cite this article: Ebrahimzadeh M, Shahabi P, Mohaddes G, Babri S, Mohammadi M, Moslem A, Mohammad-Zadeh M. Effect of Testosterone on Memory and BDNF Levels of Hippocampus in Gonadectomized Diabetic Rats. Biosci Biotechnol Res Asia 2015;12(3) |
Copy the following to cite this URL: Ebrahimzadeh M, Shahabi P, Mohaddes G, Babri S, Mohammadi M, Moslem A, Mohammad-Zadeh M. Effect of Testosterone on Memory and BDNF Levels of Hippocampus in Gonadectomized Diabetic Rats. Biosci Biotechnol Res Asia 2015;12(3). Available from: https://www.biotech-asia.org/?p=4243 |
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