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Velikanov V. I, Klyapnev A. V, Kharitonov L. V, Zenkin A. S, Kalyazina N. Yu. The Level of Metabolic and Immunological Status of Newborn Calves under The Action of Timogen on The Body of Down-Calving Cows. Biosci Biotech Res Asia 2016;13(2).
Manuscript received on : 02 April 2016
Manuscript accepted on : 20 May 2016
Published online on:  21-06-2016
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The Level of Metabolic and Immunological Status of Newborn Calves under The Action of Timogen on The Body of Down-Calving Cows

Valerian I. Velikanov1, Andrey V. Klyapnev1, Leonid V. Kharitonov2, Alexander S. Zenkin3 and Nataliya Yu. Kalyazina3

1Nizhny Novgorod State Agricultural Academy, 97 Gagarin Prospect, Nizhny Novgorod, 603107, Russian Federation.

2All-Russia Institute of Animal Physiology, Biochemistry and Nutrition, Institute township, Borovsk 249013, Kaluga Region, Russian Federation.

3Ogarev Mordovia State University,68 Bolshevistskaya str., Saransk, 430005, Republic of Mordovia, Russian Federation.

DOI : http://dx.doi.org/10.13005/bbra/2159

ABSTRACT: The purpose of the current research was to determine possible effects of timogen on accumulation of immunoglobulins and other immunogenic factors in the mammary gland of cows before calving, their release with colostrum, as well as the impact of these factors on the level of metabolic and immunological status of newborn calves after feeding them with colostrum. The data are obtained in the experiments conducted at “Arsenyevskoye” (Kaluga region) and “Mir” (Dalnekonstantinovsky district of Nizhny Novgorod region) farming enterprises. The experiment is done in the spring and summer periods of 2015 testing down-calving cows and their new-born calves. The experiment was conducted in 2 stages. The studying objects of the 1st stage were 8 down-calving cows of black-motley breed selected based on the principle of analogs, which were divided into control and experimental groups. Cows from the experimental group were exposed to intramuscular injection of timogen at a dose of 1.2 mg 3-6 days before calving. Control animals were injected subcutaneously with sodium chloride physiological solution according to the same pattern. Blood for research was taken from newborn calves one day and ten days after birth. Twenty-four hours after the start of feeding newborn calves with colostrum, elevated levels of immunoglobulins, total protein and hemoglobin were observed in their blood as compared to the calves from the control group. These differences persisted during 10 days after birth. The second stage of the experiment was conducted in the farming enterprise “Mir” of the Dalnekonstantinovsky district of Nizhny Novgorod region. The studies were performed according to the same scheme. Twelve down-calving cows were selected on the basis of the principle of analogs. Besides, blood analysis was conducted using additional morphological and immunological methods. Twenty-four hours after birth and feeding with colostrum, calves born from experimental cows, showed significantly increased number of erythrocytes and leukocytes, mainly at the cost of lymphocytes, as well as a higher level of total protein and its fractions, especially β- and γ-globulins, and increased bactericidal and lysozyme activity of blood serum.

KEYWORDS: metabolic and immunological status; down-calving cows; newborn calves; colostrum; timogen

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Velikanov V. I, Klyapnev A. V, Kharitonov L. V, Zenkin A. S, Kalyazina N. Yu. The Level of Metabolic and Immunological Status of Newborn Calves under The Action of Timogen on The Body of Down-Calving Cows. Biosci Biotech Res Asia 2016;13(2).

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Velikanov V. I, Klyapnev A. V, Kharitonov L. V, Zenkin A. S, Kalyazina N. Yu. The Level of Metabolic and Immunological Status of Newborn Calves under The Action of Timogen on The Body of Down-Calving Cows. Biosci Biotech Res Asia 2016;13(2). Available from: https://www.biotech-asia.org/?p=13168

Introduction

One of the possible methods to increase the productivity of young cattle is enhancing the level of metabolic and immunological status and resistance of the body, especially under conditions of unbalanced feeding of nursing cows and violations of the animal management technology. The main factors of the body’s protection against pathogens are antibodies, which in most animals occur in amount of about 1% of blood weight [1, 2]. Various pathological processes in the body may lead to decreased production of T- and b-lymphocytes, development of a more pronounced leukocyte migration inhibition test (LMIT), decrease the absorption capacity of neutrophils (by phagocytic number and phagocytic index), suppress T-helper and T-killer cells production, as well as the immune response mediators. This leads to an imbalance in the immune system, and veterinary professionals are faced with a new animal pathology – an immune deficiency and a decrease in the level of metabolic processes in the body. In such situations it is absolutely necessary to use immune response-modulating agents, correcting metabolic status and immune processes in the body. These agents increase the resistance of animals against adverse factors, enhance the immune response at the vaccination, activate the body defenses, and help to enhance the therapeutic efficacy in the application of antimicrobial, antiviral and antiparasitic drugs, stimulate the regeneration processes and possess growth promoting properties, have adaptogenic effects and correct the effect of stress factors on the organism [3-5].

Many drugs are offered for these purposes; however, natural substances involved in the natural regulation of body activity have the preference [2].

Previously conducted studies revealed the involvement of a number of amino acids in the regulation of digestive processes, intermediate metabolism and other metabolic processes, as well as nonspecific resistance of calves. This allowed selecting application methods of amino acids preparations for these purposes. In newborn calves, the amino acids of glycine, glutamate, taurine, and ornithine, administered orally or parenterally, increase the absorption intensity of the colostrum immunoglobulins in the intestine, and accelerate the formation of natural resistance [3, 4-7].Derivatives of amino acids, including peptides are also involved in the regulation of metabolic processes and immune status [8-11].

Thus, correction of metabolic and immune processes in the body is relevant, complex and extensive problem in humane medicine, requiring scientifically-based practical solutions. The authors sought to determine the possible impact of timogen on the ability of accumulation of immunoglobulins and other immunogenic factors in the mammary gland of cows before calving, as well as their release with colostrum that should increase the level of metabolic and immunological status of newborn calves after feeding them with colostrum.

Materials and Research Methods

For research we have chosen timogen – a synthetic peptide of thymus – glutamyl-tryptophan. Glutamic acid, which is part of it, belongs to the group of amino acids, able to accelerate the differentiation of precursors of T-cells into T-lymphocytes and to amplify the response to heterologous erythrocytes in vitro on the splenocytes that was revealed in the experiments with laboratory animals. Timogen stimulated humoral immune response in thymus-deprived mice, increased the contents of cyclic nucleotides in splenocytes, as well as the chemotaxis and phagocytosis of neutrophils, stimulated liver regeneration in rats after partial hepatectomy and in experimental cirrhosis [12-15].

It is known that the timogen increases the immunological reactivity of the organism, strengthens the lymphoid cells differentiation processes, normalizes the number of T-helpers, T-suppressors and their ratio in blood and lymphoid organs, as well as stimulates cellular metabolism and increases the animal growth rate [12-14]. The drug is prescribed in cases of immunodeficiencies, disorders of regenerative processes, for the prevention of viral and bacterial infections, to increase the immune response and animal growth rate [10, 15].

Previously, we studied the effect of timogen on the absorption of colostral immunoglobulins in newborn calves. It was found that the double intramuscular injection of the timogen preparation to down-calving cows at a dose of 100 µg at the 1st and 5-6th hours after the birth increases the level of immunoglobulins in the blood of newborn experimental calves as compared to the control animals. These differences persisted for 10 days after the birth of calves, although to a lesser extent [6, 8, 11]. To ensure a high level of metabolic and immune status in newborn calves, present studies intent to apply timogen to the pregnant cows in the last days before calving. It is known that immunoglobulins are being accumulated in the colostrum 3-9 days before calving. It is assumed that the timogen may contribute to this accumulation and thus provide the newborn calf with immunoglobulins and enhance the metabolic processes in his organism. This does not prevent the flow through the placenta of a number of substances that regulate protective factors of the fetus, as well as intake of these regulators with colostrum.

The experiments were performed on down-calving cows and their new-born calves at “Arsenyevskoye” (Kaluga region) and “Mir” (Dalnekonstantinovsky district of Nizhny Novgorod region) farming enterprises. The experimental work was conducted in the spring and summer of 2015 and consisted of 2 stages.

The first stage of the experiment was conducted at the “Arsenyevskoye” farming enterprise in Kaluga region. The studying objects were 8 down-calving cows of black-motley breed selected based on the principle of analogs. Selected cows were divided into control and experimental groups. Cows of the experimental group were exposed to intramuscular injection of timogen given as a single dose of 1.2 mg 3-6 days before calving.  The animals from the control group were exposed to subcutaneous injection of sodium chloride physiological solution according to the same pattern. Blood for research was taken from newborn calves one day and ten days after birth. The second stage of the experiment was conducted at the “Mir” farming enterprise of Dalnekonstantinovsky district of Nizhny Novgorod region. The study was conducted according to the same scheme, though a larger number of animals (12 down-calving cows) were tested as well as additional morphological and immunological methods were applied for blood analysis.

The following indicators were determined in calves during carrying out of scientific and economic experiment:

hematologic indicators included in vitro counting the number of erythrocytes and leukocytes in the Goryaev chamber, determining the amount of hemoglobin by Sahli method, the excretion of leukogram by counting in blood smears of different types of white blood cells stained by Romanovsky-Gimza;

biochemical indicators included studying the level of total protein, blood urea and glucose in the serum of animals by means of Olympus AU480 biochemical analyzer;

immunologic indicators included determining immunoglobulins on the Minicap and Sebia analyzer, determining bactericidal activity of blood serum using Staphylococcus epidermidis test culture modified according to Smirnov and Kuzmina (1966) as well as lysozyme activity, using a Micrococcus lysodeikticus test culture in the modification of USRIEVM (Ukrainian Scientific-Research Institute of Experimental Veterinary Medicine).

The analyses were performed in the “Hemohelp” laboratory of the Department on “Anatomy, Surgery and Internal Non-communicable Diseases” at Nizhny Novgorod State Agricultural Academy, and in the laboratory of protein-amino acid nutrition of All-Russia Institute of Animal Physiology, Biochemistry and Nutrition.

The resulting digital material was subjected to statistical processing using conventional parametric methods. The degree of confidence was determined by Student’s t-test employing the Microsoft Excel (2000) software package and STAT 3 software program.

Discussion and Results

Analyzing the data obtained in the 1st stage of the experiment, it is noted that a day after the start of feeding calves with colostrum, the level of immunoglobulins in the blood of newborn calves has increased by 32.4%, total protein – by 5.6%, and hemoglobin – by 3.9% as compared with the calves in the control group, where nursing cows were not administered timogen. These differences were preserved to a certain extent 10 days after calves birth as well. Hematological parameters were statistically processed, summarized and presented in Table 1.

Table 1: Hematological parameters of calves’ blood

Indicator Twenty-four-hours old calves Ten-days-old calves
control experiment control experiment
Immunoglobulins, mg/ml 10.8±1.2 14.3±1.1* 11.7±0.9 13.8±1.3
Total protein, g/l 57.1±4.3 60.3±5.6 59.2±6.1 61.5±4.7
Hemoglobin, g/l 89.2±7.6 92.7±9.4 92.3±7.9 94.2±8.5
Blood urea, mmol/l 4.9±0.5 5.2±0.4 4.8±0.4 4.5±0.3
Glucose, mmol/l 5.6±0.5 5.9±0.5 5.3±0.5 5.2±0.5

*≤0.05 as compared to control

Average daily gain of live weight of calves from the experimental group after two-month rearing amounted to 472 g/day versus 413 g/day in the control calves; that is, the increase amounted to 14.3%.

Analyzing the data obtained in the 2nd stage of the experiment, it was noted that one day after birth and feeding calves with colostrum, the calves born from cows in the experimental group, have shown insignificant increase of the number of erythrocytes (by 16.1%) and leucocytes (by 15.9%) in blood, as well as decrease in the number of monocytes in comparison with control animals. At that, the number of leukocytes was higher mainly due to lymphocytes, and to a lesser extent due to the number of neutrophils. Hematological parameters of calves’ blood in the 2nd stage of the experiment were statistically processed, summarized and presented in Table 2.

Table 2: Hematological parameters of calves’ blood (2nd stage of the experiment)

Indicator Twenty-four-hours old calves Ten-days-old calves
control experiment control experiment
Erythrocytes, million/µl 5.0±0.4 5.8±0.4 4.9±0.3 5.49±0.5
Leukocytes, thousand/µl 4.6±0.2 5.3±0.3 4.7±0.4 5.22±0.4
Banded neutrophil, % 2.1±0.1 3.0±0.4 2.7±0.3 2.9±0.2
Segmentonuclear neutrophils, % 27.3±1.9 24.8±3.2 26.1±2.5 25.3±2.8
Eosinophils, % 0.8 1.2 1.0 1.8
Monocytes, % 4.0 0.8 3.6 2.8
Lymphocytes, % 68.8±4.9 70.2±5.5 66.5±4.3 67.2±7.2
The total number of neutrophils, thousand/µl 1.6 1.5 1.4 1.4
The total number of lymphocytes, thousand/µl 3.0 3.7 3.1 3.5
The ratio of lymphocytes/segmentonuclear  neutrophils 2.4 2.8 2.6 2.7
The ratio of neutrophils/lymphocytes 0.5 0.4 0.3 0.4

*≤0.05 compared to control

Apparently, a change in the content of neutrophils and lymphocytes has led to a change of nonspecific reactivity indicators. Thus, the index of lymphocytes/segmentonuclear neutrophils has increased by 29%, while index of  neutrophils/lymphocytes has decreased  by 11.1%.

After twenty-four hours from starting feeding with colostrum, the calves born from cows, which were subjected to intramuscular injection of timogen at a dose of 1.2 mg per animal once a day 3-6 days before calving, have shown a higher level of total protein (by 5.5%) and its fractions β- and γ-globulins in blood, which exceeded control by 9.9 and 17.7%, respectively. Bactericidal activity of blood serum, reflecting the cumulative effect of humoral and cellular protection components, was significantly above controls in calves of the experimental group (by 9.6%), at that lysozyme activity increased by 8.7%.

The revealed differences of morphological indicators of blood in calves involved in the 2nd stage of the experiment were reflected in biochemical and immunological parameters of blood of these animals and largely preserved during 10 days after birth. The observed change in blood indicators of calves in the experimental group is probably associated with revealed high content of immunoglobulins in colostrum of cows, which were administered timogen before calving. Thus, in experimental calves their level amounted to 52.1 mg/ml versus 43.2 mg/ml in control, while lymphocytes exceeded control by 7.2%. However, intake with colostrum of larger quantities of other factors enhancing pinocytosis in the gut of the calves from experimental group cannot be excluded. Immunological and biochemical parameters of blood are statistically processed, summarized and presented in Table. 3.

Table 3: Immunological and biochemical parameters of calves’ blood (2nd stage of the experiment)

Indicator Twenty-four-hours old calves Ten-days-old calves
control experiment   control
Hemoglobin, g/l 95.8±8.5 99.7±7.6 94.7±8.1 97.9±9.1
Blood urea, mmol/l 5.4±0.6 5.6±0.5 5.2±0.5 5.3±0.4
Glucose, mmol/l 6.1±0.4 5.9±0.4 5.9±0.4 5.5±0.6
Total protein, g/l 57.6±4.6 60.8±3.7 58.9±5.3 61.7±4.1
Protein fractions: albumins, g/l 18.5±1.1 17.8±1.5 20.0±1.6 19.3±1.3
α-globulins, g/l 16.4±1.3 15.6±1.2 15.1±0.9 14.2±1.5
β–globulins, g/l 9.3±0.5 10.8±0.9 9.1±1.3 10.3±1.0
γ–globulins, g/l 13.4±0.8 16.6±0.7* 14.8±1.5 17.7±1.8
Bactericidal activity, % 36.3±2.8 39.8±4.2 38.5±3.4 40.7±2.9
Lysozyme activity, % 17.3±1.9 18.8±1.5 18.6±2.0 19.4±1.6

*≤0.05 compared to control

Stimulation of immunoglobulins and leucocytes separation out of colostrum as well as changes in other immunogenic factors, have contributed to the increase in the level of metabolic and immunological status of calves. This has led to the decrease of their morbidity and increase in weight gain by 17.6% as compared to the control, i.e. by 537 and 458 g/day in the experimental and control groups, respectively, in the dairy cultivation period during two-month observation.

Conclusion

Correction of metabolic and immune processes in the body is a relevant, complex and extensive problem in humane medicine, which requires scientifically-based practical solution.

A single intramuscular injection of the timogen aqueous solution to down-calving cows 3-6 days before calving contributed to the accumulation in the mammary gland of immunoglobulins and other immunogenic factors. The amount of immunoglobulins in the colostrum of the tested cows, equal to 52.1 versus 43.2 mg/ml and a number of lymphocytes greater at 7.2%, helped to improve immunological status and metabolic processes in the organism of newborn calves after feeding them with colostrum that was clearly reflected by hematological status. A numbers of red blood cells and white blood cells were increased mainly due to lymphocytes, as well as a higher level of protein and its fractions, especially β- and γ- globulins were observed compared to the control. Also tested calves had higher bactericidal and lysozyme activity of blood serum.

It is found in general that the injection of timogen to down-calving cows contributes to the accumulation of immunoglobulins and other immunogenic factors in the mammary gland of cows before calving and their release with colostrum that promotes the formation of high-level metabolic and immunological status of newborn calves after feeding them with colostrum.

The data obtained allow clarifying some aspects of immune status and metabolism regulation in the organism of newborn calves that must be taken into account when developing physiologically sound practical ways of immunomodulation and improvement of metabolic processes in calves organism during the initial period of nurturance, which is often accompanied by immunodeficiency and diseases of the gastrointestinal tract of these animals.

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