Introduction

Artemisia is a huge diverse genus with shrubs and small herbs mostly  found in northern temperate areas. The secondary metabolites and essential oils of Asteraceae family especially Artemisia species  are valued for their essential oils and the remarkable medicinally. For many years, the Essential oils of Artemisia have been widely produced for medicinal purposes1. Different types of Artemisia have been described for their organic exercises. It is considered to deliver most restoratively essential optional metabolites.^2,3

A few intriguing investigations utilizing Artemisia spp. demonstrated a progression of antimicrobial and cancer prevention agent activities.^4,5,6,7

Restorative plants are Nature’s blessing to individuals to enable them to seek after an ailment free solid life, and along these lines can assume a critical part in saving wellbeing. Plants have been utilized as medications by people since a huge number of years back. Today, all the world’s societies have a broad learning of home grown drug. Customary solution depends on convictions and practices that existed before the improvement of purported “present day pharmaceutical” or “logical medication treatment”. These practices are a piece of a nation’s social legacy and are transmitted orally or by composed transmission Artemisia monosperma is appropriated in various Arab nations forsake, for example, Saudi Arabia and Iraq.⁸ Artemisia monosperma is rumored in conventional drug for its antispasmodic, anthelmintic and hostile to hypertensive properties.^9,10,11 Artemisia monosperma answered to contain a few phytochemicals, for example, unpredictable oil,¹² ûavonoids,¹³ alkaloids¹⁴ and coumarins.¹⁵ Artemisia argyi is a herbaceous enduring plant with a crawling rhizome. It is local to China, Japan and the far eastern parts of the previous Soviet Union. It is utilized as a part of natural pharmaceutical for states of the liver, spleen and kidney.¹⁶ This examination planned to explore the efficacies of fluid concentrates of Artemisia species developing in Saudi Arabia against some human pathogenic microscopic organisms.

Materials and Methods

Samples of Plants

The plants (Artemisia monosperma, Artemisia cina and Artemisia argyi) were collected from different locations of Saudi Arabia in the spring of 2016G, therefore, identified by Herbarium taxonomists of Botany and Microbiology Department, Faculty  of Science, the University King Saud (KSU). According to standardized procedure of the Botany and Microbiology Department, the plants were identified.

Preparation of Aqueous Extracts of Artemisia Species

The Cutting into small pieces, grinding and soaking of the leafs plants (Artemisia monosperma, A. cina and A. argyi) were perform respectively. The soaked was rocked for 24 hours after diluted with distilled water to make 1: 3 (weight/volume). Then, a household blender was used, for one min at maximum speed, to homogenize the mixture. Then, using double layer of cheesecloth, the mixture was filtered.  The filtered mixture was then centrifuged at 3000xg for 10 minutes with could temperature (4 °C). MF-Millipore Membrane Filter (0.45µm) was used to sterilize the supernatant liquid. This supernatant was stored at -20 °C for later use and referred to as aqueous extract.

Bacterial Isolates

The following Gram positive and Gram negative bacteria isolates were used throughout this study; E. coli 25696 , Staph. aureus S9597, Ps. aeruginosa 22209, Entero. faecalis S10249. The pathogenic bacterial strains were obtained from Riyadh Hospital (KSU), Saudi Arabia; therefore, four bacterial strains were used throughout this study.

Mueller-Hinton or/and blood medium agar were used to maintain the strains by sub-culturing on a 10-mm thick layer agar. All samples were incubated for 24 hours at 37°C. The same procedure was used for growth on Luria Bertani (LB) broth medium or/and Nutrient Broth medium. For long-term preservation, the bacteria were suspended in sterile 25% glycerol in sterile Tryptone Soya Broth and kept at 4°C or -80°C.

Minimum Inhibitory Concentration (MIC)

The broth media dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract of Artemisia monosperma, Artemisia cina and Artemisia argyi. The final concentration of the extract was with mg/ml in brain heart infusion broth were inoculated with 100 µl of strains and were incubated at 37° C for 24 hour. The MIC was defined as the lowest concentration (mg/ml) of the extract resulting in clear broth media while minimum bactericidal concentration (MBC) was defined as the lowest concentration (mg/ml) of the extract resulting in no growth of bacteria after culture on agar media.

Results and Discussion

The after effects of antibacterial action are given in the Table 1 and 2, which plainly demonstrate that every one of the concentrates at different focuses have indicated antibacterial action of against the whole tried creatures. Watery, water removes have indicated better action of all the four microorganisms. Fluid concentrate was more compelling against E.coli and less activity of E. faecalis. Comparative investigations somewhere else recorded antibacterial action of seed removes against S. aureus, S. epidermidis, E. coli, and P. aeruginosa17.

Restorative plants keep on playing a focal part in the human services frameworks of extensive extents of the total populace, especially in creating nations, where home grown medication has a long and continuous history of use18. The present results are in agreement with Barbour et al. (2004)19, Motavalizadehkakhky et al. (2013)20, Moglad et al. (2012)21, and Entezari et al.  (2009)22  with  differing  degrees  of  intensity.

Table 1: Minimum inhibitory concentration (MIC) of aqueous extracts of Artemisia species against some pathogenic bacteria

Medicinal plants continue to play a central role in the healthcare systems of large proportions of the world’s population, particularly in developing countries, where herbal medicine has a long and uninterrupted history of use.¹⁸  The present results are in agreement with Barbour et al. (2004),¹⁹ Motavalizadehkakhky et al. (2013),²⁰ Moglad et al. (2012)²¹ and Entezari et al. (2009)²² with varying degrees of potency. The difference in potency may be due to the stage of collection of the plant sample, soil nature, other environmental factors, storage conditions, the part of plant used, method of extraction, method of screening, concentration of extract, and different sensitivity of the tested strain. In the present study, plant extract possess antibacterial activity against tested organisms. This clearly indicates that these extracts might have different potential activity.

Table 2:  Minimum bactericidal concentration (MBC) of aqueous extracts of Artemisia species against some pathogenic bacteria

The distinction in intensity might be because of the gathering phase of the plant sample, soil nature, other environmental factors, storage conditions, the part of plant used, method of extraction, method of screening, concentration of extract, and different sensitivity of the tested strain. In the present study, plant remove have antibacterial movement against tried life forms. This clearly indicates that these extracts might have different potential activity.

The search for new antimicrobial agents has been necessitated by the increase in antimicrobial resistance in recent years. Fifty thousand people die every day worldwide due to infectious diseases.²³ Natural plant products are a good source of new antimicrobials as they generally have low toxicity, cause minimal environmental pollution, have a low risk of development of resistance by pathogens,²⁴ are cheap, and are generally safer than synthetic medicines.²⁵ The use of known medicinal plants is advantageous as they have been prescreened over thousands of years, resulting in a higher probability of isolating useful and safe compounds from them than from plants not in use by humans already.²⁶ Plants materials are either present in or have provided models for approximately 50% of drugs25. Screening Artemisia species for antibacterial activity was more likely to be successful as these plants are used for medicinal purposes.

The extracts from the three plants were generally more effective against E.coli, Ps.aeruginosa than Staph. aureus and Entero. faecalis. E. coli, Ps. aeruginosa  are Gram-negative bacterium while Staph. Aureus and Entero.faecalis are Gram-positive, suggesting that the plant extracts are more efeffective against Gram-negative than Gram- positive bacteria. Finally, It is presumed that the plant remove have antibacterial action against tried life forms. The antibacterial movement of the plants might be because of the nearness of different dynamic standards in their takes off. Additionally ponders are expected to seclude and portray the bioactive standards to grow new antibacterial medications.

References

1. Abdul R., Waheeta H. Antibacterial activity of Artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria. BMC Complemet. Alternat. Medici. 2010;10:1-6.
2. Ambasta S. P. (ed): The useful plants of India. Publications & Information Directorate. CSIR, New Delhi. 1986;55-56.
3. Priscila I. U., Mariama T. S., Luiz C. S., Luciano B., Fernandes A. J. Antibacterial activity of medicinal plant extracts. Braz. J. Microbiol. 2007;38(5):717-719.
4. Juteau F., Masotti V., Bessiere J. M., Dherbomez M and Viano J. Antibacterial and antioxidant activities of Artemisia annua essential oil. Fitoterapia. 2002;73:532-535.
   CrossRef
5. Kordali S., Kotan R., Mavi A., Cakir A., Ala A., Yildirim A. Determination of the Chemical Composition and Antioxidant Activity of the Essential Oil of Artemisia dracunculus and of the Antifungal and Antibacterial Activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera Essential Oils. J. Agric. Food Chem. 2005;53:9452-9458.
   CrossRef
6. Kordali S., Cakir A., Mavi A., Kilic H., Yildirim A. Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three turkish Artemisia Species. J. Agric. Food Chem. 2005;53:1408-1416.
   CrossRef
7. Curini M., Epifano F., Genovese S. Tammaro F., Menghini L. Composition and antimicrobial activity of the essential oil of Artemisia dracunculus piemontese from Italy. Chem. Nat. Comp. 2006;42:738-740.
   CrossRef
8. Danin A. Contributions to the flora of Jordan 3. A new species of Artemisia (Compositae, Anthemideae) from S. Jordan. Willdenowia. 1999;29:147-153.
   CrossRef
9. Sharaf A., Fahmy I. R., Ahmad Z. F., Abdel- Moneim F.  Pharmacological study of Artemisia monosperma. Egypt. Pharm. Bull. 1959;41:47-52.
10. Chakravarty H. Plant Wealth in Iraq, Vol. I, Ministry of Agriculture, Iraq, Baghdad. 1976;43–45.
11. Wagner H.,Wolff P. New Natural Products and Plant Drugs with Pharmacological, Biological or Therapeutical Activity. Springer-Verlag Berlin Heidelberg, New York. 1977;184-192.
12. Saleh M. A. Volatile components of Artemisia monosperma and Artemisia judaica growing in the Egyptian deserts. Biochemi. Systemati. Ecolo. 1985;13(3):265-269.
    CrossRef
13. Elgamal M., Ouf S., Hanna A., Yassin F. Phytochemical and mycological investigation of Artemisia monosperma. Folia Microbiologica. 1997;42:203–210.
    CrossRef
14. Zaki D.,Aziz M. A., El-Gengeihy S., Morsi N. Antimicrobial potentiation of some Egyptian desert plants. Herba Hung. 1984; 23: 73-84.
15. Hammoda H. M., Shawky E., Kinoshita E., Takayama H. Phytochemical and biological investigation of Hymenocallis littoralis SALISB. Chem Biodivers. 2008;5:332-340.
    CrossRef
16. Otsuka K., Shoji J., Takido M., Cho S. A Pictorial Encyclopedia of Chinese Medical Herbs Series (I) Chuokoran-Sha Inc.: Tokyo, Japan. 1992;228.
17. Salar R. k., Suchitra. Evaluation of antimicrobial potential of different extract of S. xanthocarpum. Aft. J. of Micr. Res. 2009;3(3):97-100.
18. Koduru S., Grierson D. S., Afolayan A. J. Ethno botanical information of medicinal plants used for treatment of cancer in the Eastern Cape Province, South Africa. Curr. Sc., 2007; 92(7):906-908.
19. Barbour E. K., Al Sharif M., Sagherian V. K., Habre A. N., Talhouk R. S., Talhouk S. N. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J. Ethnopharm. 2004;93(1):1-7.
    CrossRef
20. Motavalizadehkakhky A., Shafaghat A., Zamani H. A., Akhlaghi H., Mohammadhosseini M., Mehrzad J., Ebrahimi Z. Compositions and the in vitro antimicrobial activities of the essential oils and extracts of two Achillea species from Iran. J. Med. Plant Res. 2013;7(19):128,0–1292..
21. Moglad E. H. O., Alhassan M. S., Koko W. S., Saadabi A. M. In vitro antimicrobial activity of Sudanese medicinal plants. J. Med. Sci. 2012;12(7):219-223.
    CrossRef
22. Entezari M.,  Hashemi  M.,  Ashki  M., Ebrahimian S., Bayat M.,Saraji A. R. A., Rohani S. R. Studying the ef fect Echinophora platyloba extract on bacteria (Staphylococcus aureus and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus flavus and Aspergillus niger) in vitro. World J. Med. Sci. 2009;4(2):89-92.
23. World Health Organization, Infectious Diseases Kill over 17 Million People a Year: WHOW arns of Global Crisis,WHO. Indian  Pediatr. 1996;33(7):617-23.
24. Mbega E. R., Mortensen C. N.,  Mabagala R. B., Wulff E. G.  The effect of plant extracts as seed treatments to control bacterial leaf spot of tomato in Tanzania.J. of Gene. Plant Patholo. 2012;78(4):277–286.
25. Ravikumar S., Ali M. S. A., Ferosekhan M. Antibacterial activity of chosen mangrove plants against bacterial specified pathogens. World Appl. Sci. J. 2011;14(8):1198–1202.
26. Nielsen T. R. H.,  Kuete  V.,  Jager A. K., Meyer J. J. M., Lall N. Antimicrobial activity of selected South African medicinal plants. BMC Complemen. Alter. Medi. 2012;12:74.
    CrossRef