Introduction

The strain Cellulosimicrobium aquatile 3bp was recently described as a novel species of the genus Cellulosimicrobium. The cells are Gram-stain positive, non-spore forming, non-motile, yellow-pigmented, rod shaped and coccoid aerobic in nature. The species showed positive for catalase activity and oxidase activities (Sultanpuram et al., 2015).

Sequencing of the type strain is part of Genomic Encyclopedia of Type Strains, Phase III: the (KMG-III) project (Whitman et al., 2015). Project information is deposited in the Genomes on Line Database (GOLD) (Reddy et al., 2015), and the high quality draft genome sequence is deposited in GenBank and in the Integrated Microbial Genomes database (IMG) (Markowitz et al., 2014). Draft sequencing, initial gap closure and annotation were performed by the DOE Joint Genome Institute (JGI) using state-of-the-art sequencing technology (Mavromatis et al., 2015).

Materials and Methods

An Illumina standard shotgun library was constructed and sequenced using the Illumina HiSeq 2500-1TB. Illumina paired-end reads of average 250-bp length and total read size of ~ 548 Mbp (clean data) were assembled using Velvet (version 1.1.04)(Zerbino and Birney, 2008), simulated paired end reads were created from Velvet contigs using wgsim and simulated read pairs were reassembled using Allpaths-LG (version r42328) (MacCallum et al., 2009). Protein-coding genes were identified using Prodigal (Hyatt et al., 2010), as part of the DOE-JGI genome annotation pipeline (Huntemann et al., 2015). Additional gene prediction analysis and manual functional annotation were performed within the Integrated Microbial Genomes (IMG) platform, which provides tools for analyzing and reviewing the structural and functional annotations of genomes in a comparative context (Markowitz et al., 2014). Genome annotation procedures are detailed in Markowitz et al. (2014) and references therein. Briefly, the predicted CDSs were translated and used to search the NCBI nonredundant database, UNIProt, TIGRFam, Pfam, KEGG, COG and InterPro databases. Transfer RNA genes (51 in number) were identified using the tRNAScan-SE tool and other noncoding RNAs (8 in number) were found using INFERNAL.

Results and Discussions

The assembled draft genome contained 4,317,712 bps with 74.73 % GC content. The analysis of the draft genome of Cellulosimicrobium aquatile 3bp predicted 3796 protein coding sequences (CDS) of which 77.97 % were assigned to recognized functional genes. Ribosomal RNA genes (4 in number) were predicted using hmmsearch against the custom models generated for each type of rRNA.

The genome also harboured a cluster of genes coding for glucosidase activities. These genes include, β-glucosidase (770 aa; locus tag BXA05_RS01230; accession number FTM101000001), glucan, 1,3-β-glucosidase (526 aa, locug tag BXA05_RS01565; accession number FTM1000001), β-glucosidase (997 aa; locus tag BXA05_RS03520; accession number FTM101000001), β-glucosidase (391 aa; locus tag BXA05_RS04915; accession number FTM101000002), α-glucosidase (588 aa; locus tag BXA05_RS15140; accession number FTM101000006), β-glucosidase (516 aa; locus tag BXA05_RS15215; accession number FTM101000006) and α-glucosidase (496 aa; locus tag BXA05_RS15890; accession number FTM101000006). This draft genome sequence will further help in understanding the genetic potential of Cellulosimicrobium aquatile for glucosidase activity.

Nucleotide Sequence Accession Numbers

The draft sequence of C. aquatile strain 3bp obtained in this Whole Genome Shotgun project has been deposited at GenBank under the accession no. FTMI00000000. The Genomes on Line Database (GOLD) ID is PRJEB18885.

Acknowledgments

We are thankful to Prof. William Whitman for proposing the genomic sequencing of this organism under KMG-III. This organism was isolated and described in Department of Science & Technology, Government of India, FTSYS project grants (SB/FT/LS-115/2012-13 and SB/FT/LS-320/2012-13, respectively).

Conflicts of Interest

The authors declare no conflicts of interest.

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