Manuscript accepted on : 19-11-2022
Published online on: 24-11-2022
Plagiarism Check: Yes
Reviewed by: Dr. C.P. Sreena
Second Review by: Dr. Robert Susło
Final Approval by: Dr. Eugene A. Silow , Dr. Prof. Imran Ali
Diversity and Extracellular Enzyme Production of Fungal Endophytes from the Genus Ocimum L.
Department of Botany, Government Victoria College (Affiliated to university of Calicut), Palakkad, Kerala – 678001, India
Corresponding Author E-mail: rasmibotany@gmail.com
DOI : http://dx.doi.org/10.13005/bbra/3060
ABSTRACT:
Ocimum tenuiflorum, O. gratissimum, and O. basilicum are medicinal plants extensively used in the traditional medicine of Kerala. The study is aimed at investigating the endophytic mycoflora associated with these Ocimum species and their ability to produce enzymes in vitro. A total of 149 fungal endophytes were isolated from roots, stems, and leaf segments from July to November 2021. They were grouped into 27 morphotypes, including five non-sporulating taxa. The highest number of isolates were obtained from the plant O. basilicum. An equally lower number of isolates were obtained from O. gratissimum and O. tenuiflorum. A greater number of fungal endophytes were obtained from the leaf segments of O.basilicum and least number of isolates obtained from the leaf segments of O.gratissimum. Isolates of Aspergillus niger complex, Diaporthe sp., and Daldinia eschscholtzii showed the highest colonizing frequency. In vitro analysis for enzyme production by all morphotypes was done and, except for laccase, all tested enzymes showed positive results.
KEYWORDS:
Diversity; Enzyme Activity; Ocimum plants
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Introduction
Fungal endophytes are a heterogeneous group of organisms, mainly belonging to Ascomycotina and Deuteromycotina. Petrini ¹ defined endophytes as organisms which colonize symptomless in the internal tissues of host plant at some stages of their life cycle. The interaction between endophyte and host depends on the mode of infection and the defensive reaction of the host plant. The colonization could be local or systemic, inter- or intracellular 2. In endophytic association, the interaction between the host and fungal partner depends on three factors: the aggressiveness of fungi, the developmental stage of both host and fungi, and the host plant’s susceptibility to pathogenicity. so that the symbiotic nature of endophytic relations is in a continual flux. They may enter into mutualistic symbiosis or pathogenicity 3 .
Fungal endophytes that were associated with their host plants influenced them. They help to increase fitness by conferring abiotic and biotic stress tolerance, reducing water consumption, increasing overall biomass, etc.4 .The ability of endophytes to produce secondary metabolites and enzymes is directly correlated with their ecological importance. Endophytes produce xylan, pectin, peroxidases, laccases, cellulase, hemicellulose, and other enzymes necessary for effective colonization5. The structure and functional properties of bioactive secondary metabolites produced by fungal endophytes were comparable to those of secondary metabolites produced by plants. As a result, endophyte-derived metabolites are widely studied6. Important compounds obtained from fungal endophytes include vincristin, podophyllotoxin, camptothecin, naphthodianthrones, and palcitaxol etc.7,8,9,10,11.
The genus Ocimum, which has high therapeutic potential, belongs to Lamiaceae family. They are commonly used in traditional Ayurvedic and Unani medicine 12. The high medicinal effect of Ocimum species is due to their richness in secondary metabolites, the majority of which are essential oils. Ocimum plants have been studied pharmacologically and have been found to have antiviral, larvicidal, antinociceptive, anti-inflammatory, antipyretic, antibacterial and antifungal activities. They are frequently employed in the treatment of blood related illnesses, respiratory conditions, feverish illness, nausea, migraine, abdominal cramps, gonorrhoea, dysentery, headache, dizziness, piles, cough, paralysis, nervous temperament, muscle cramps, insect bites, diabetes 13,14.
The variety of fungi that live inside the Ocimum species and their capacity for bio-prospecting have been the subject of extensive research in recent years. Numerous endophytic fungi, such as Colletotrichum sp., Fusarium sp., Alternaria sp., Penicillium sp., Nigrospora sp., etc., are frequently found in association with Ocimum plants 15,16,17. Rajagopal et al. 17 reported a high number of fungal isolates from O. basilicum and O. tenuiflorum, representing greater diversity of their endophytes compared to other plants (Coleus aromaticus and Tridax procumbens), thus lending credence to our premise that these Ocimum species are rich in fungal endophytes and that it is important to investigate their biological potential.
In Kerala, Ocimum tenuiflorum, Ocimum gratissimum, and Ocimum basilicum are quite common and extensively dispersed. Although the diversity of endophytic fungi has been explored, there are no investigations on their capacity to produce extracellular enzymes. Endophytic fungus produces a variety of enzymes that have a significant impact on the food, pharmaceutical, and other industries. These enzymes are utilized in many different industries, including as pulp and paper production, textile production, brewing, laundry, and food processing. This study aims to describe the diversity of fungal endophytes present in the roots, stems, and leaf segments of O. tenuiflorum, O. basilicum, and O. gratissimum. Additionally, it evaluates their ability to produce extracellular enzymes including tyrosinase, amylase, cellulase, and asparaginase as well as other enzymes like protease and laccase.
Material and Methods
Sample collection
Roots, stem, and leaves of O. tenuiflorum, O. basilicum, and O. gratissimum plants were sampled for the isolation and investigation of endophytic fungal communities. The study was conducted during the period July to November 2021. Three randomly selected locations in the Palakkad district of Kerala, India (10°47′10′′ N, 76°39′6′′ E; 11°0′26′′ N, 76°23′57′′ E; and 10°47′35′′ N, 76°21′52′′ E) were sampled. From each site, a total of nine plants were collected (three plants of each species), and from each plant, three segments of root, stem, and leaves were used for isolation. All collected samples were kept in sterilized Ziplock plastic bags and placed into Potato Dextrose Agar (PDA) medium within six hours of being collected.
Surface sterilization and isolation
Before isolation, the samples were subjected to surface sterilization. The plant material was rinsed in running tap water to remove dust and debris for 2 to 3 minutes. After clear washing, the samples were cut into small pieces and further processing was done under aseptic conditions. surface sterilization is performed by the immersion of plant segments in sequence of ethanol, diluted sodium hypochlorite, and ethanol (each set of plant material was treated with 70% ethanol for 1 minute followed by immersion in 4% sodium hypochlorite for 3 minutes and again in 70% ethanol for 30 seconds). Lastly, the segments were rinsed three times with sterile (autoclaved) distilled water 5. All surface-sterilized segments were dried on sterile blotting paper. After proper drying, each plant segment was placed onto sterilized PDA media supplemented with 50 mg/L ampicillin to prevent bacterial contamination. Petri plates were incubated for 4 to 7 days at 25±1°C. As fungal hyphae emerged, they were sub cultured and maintained on freshly prepared PDA slants. Colonization frequency and isolation rate were also recorded using the following formula 18.
Identification of fungal endophytes
All endophytic isolates obtained were characterized morphologically by analyzing the characteristics of the fungal colony and spores and the identification confirmed by available mycological literature 19,20,21.
Molecular analysis was performed to confirm identification of isolates showing the highest activity in each type of enzyme, we adapted the procedure of White et al.22 with minor modification. The DNA was isolated by using the NucleoSpin® Plant II Kit (Macherey-Nagel). The sequencing reaction was done in a PCR thermal cycler (GeneAmp PCR System 9700, Applied Biosystems) using the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA) following the manufacturer’s protocol. The primers used were forward ITS1(F)-5′-TCCGTAGGTGAACCTGCGG-3′ and reverse ITS-4(R)3′-TCCTCCGCTTATTGATATGC-5′. The Sequence Scanner Software V1 (Applied Biosystems) was used to check the sequence quality. Sequence alignment and required editing of the obtained sequences were carried out using Bioedit. Online BLAST searches were conducted on the GenBank sequence database (http://www.ncbi.nlm.nih.gov/BLAST/). The megablast ITS rDNA queries identified GenBank accessions with E-values of zero.
Enzyme Activity
All fungal endophytic morphotypes were inoculated with appropriate broth at 25±1 °C to analyse extracellular enzyme production. In this study, we considered only amylase, cellulase, protease, asparaginase, tyrosinase, and laccase for evaluating the enzyme production by fungal endophytes. The diameter of the hydrolysis and the fungal colony were measured after 5 to 7 days of incubation at 25±1 °C, and the enzyme index was calculated 23. All experiments were done in triplicates.
Enzyme index = Diameter of hydrolysis / Diameter of fungal colony.
Asparaginase
The cultures were inoculated into a modified Czapex Dox agar medium containing 2.0 g/L glucose, 1.52 g/L KCl, 0.52 g/L MgSO4.7H2O, 0.001 g/L ZnSO4.7H2O, 0.001 g/L FeSO4.7H2O, and 16 g/L Agar with 10.0 g/L L-asparagine. Phenol red solution (3ml) were added to the media before sterilization. Asparaginase activity was indicated by the formation of a pink colour in colourless media 24.
Amylase
The cultures were inoculated on to autoclaved Glucose Yeast Extract Peptone (GYP) agar (1 g/L glucose, 0.1 g/L yeast extract, 0.5 g/L peptone, 16 g/L agar, pH 6) supplemented with 2% soluble starch to detect amylase activity. Following incubation, the plates were flooded with a freshly prepared iodine solution. A white or clear halo indicates amylase activity 25.
Cellulase
Cellulase activity was detected by inoculating each culture onto the GYP agar with 0.5% sodium carboxymethyl cellulose (CMC). After the incubation period, the cultures were flooded with 0.2% Congo red, washed with 1 M sodium chloride (NaCl), and allowed to stand for 15 min. The appearance of a yellow halo around the colony signifies that cellulase has been produced 25.
Laccase
Cultures were inoculated on to GYP agar medium with 0.005% of 1-naphthol. By laccase enzyme oxidation of 1-naphthol, colourless media will be changed to blue 26 .
Tyrosinase
Cultures were incubated with GYP medium to test for tyrosinase activity. The colony was overlaid with 0.11% p-cresol and 0.05% glycine and left for 24 hours. Positive tyrosinase activity can be detected by a reddish-brown zone surrounding the colony25 .
Protease
The proteolytic activity of the isolates was determined by growing them on GYP medium supplied with 0.4% gelatin. Separately, 8 g of gelatin in 100 mL of distilled water was added to the sterilized media 26. Following incubation, the culture was flooded with saturated aqueous ammonium sulphate. Protease positive activity was observed around the colony in the form of a clear zone.
Results and Discussion
A total of 149 endophytes were isolated from 243 segments of three Ocimum species studied. Among this isolates, 39 cultures were obtained from O.tenuiflorum, 71 fungal isolates from O.basilicum and 39 fungi were obtained from O,gratissimum (Table 1). The highest numbers of isolates were obtained from the leaves of O. basilicum, stems of O. tenuiflorum and roots of O. gratissimum. This suggests that the isolation rate is not purely dependent on the tissues but may be the function of fungal taxa involved. In contrast to this result, Banerjee et al.27 showed that a high number of fungal endophytes were obtained from O. tenuiflorum as compared to O. basilicum.
Table 1: Total number of fungal isolates obtained from Ocimum tenuiflorum, O,gratissimum and O.basilicum
Host plant |
Root segment |
Stem segment |
Leaf segment |
O.tenuiflorum |
12 |
14 |
13 |
O,gratissimum |
18 |
15 |
6 |
O.basilicum |
18 |
22 |
31 |
Based on morphological features such as colony characters and conidial and spore characters, all 149 fungal isolates were separated and grouped into 27 morphotypes, with 22 sporulating and 5 non-sporulating cultures. Among them, five morphotypes belonged to Xylariales and Hypocreales each, three morphotypes belonged to Eurotiales, Diaporthales, and Botryospheriales each, two morphotypes come under the order Pleosporales and Glomerellales with one morphotype. The highest number of non-sporulating isolates was obtained from the stem segments of O. tenuiflorum. From O. basilicum, only a non-sporulating isolates was obtained, which was isolated from leaf segments, and from O. gratissimum, also only one isolate was obtained and it was recovered from both root and stem segments.
The highest colonization frequency was obtained from Aspergillus niger complex with 44.4%, which was isolated from all host plant and root and stem segments. Diaporthe sp. (culture code: MBLC2), showed 25.9% of colonization frequency which also had occurrence in all host plants, but they were obtained only from leaf segments. The third highest colonization frequency (18.5%) was reported with Daldinia eschscholtzii obtained from the stem segments of O. gratissimum (Table. 2). Earlier studies reported that, species of Aspergillus and Diaporthe were the most dominant endophytes, with a high rate of colonization 16,28,29 .
Present study also reflect the same, along with Daldinia eschscholtzii. Isolates of Aspergillus niger complex showed more dominance over the others and they isolated from stem and root segments of all host plants under study. Aspergillus sp. are well known for their pathogenicity 30, but previous studies revealed that, Aspergillus sp. can occur as endophytes with high bioprospecting ability 31,32,33.
Table 2: Details of morphotypes obtained from Ocimum species
|
COLONIZATION FREQUENCY (CF) (%) |
Total CF |
||||||||||
ORDER |
CULTURE CODE |
NAME |
O.tenuiflorum |
O.basilicum |
O.gratissimum |
|||||||
Root |
Stem |
Leaves |
Root |
Stem |
Leaves |
Root |
Stem |
Leaves |
|
|||
Xylariales
|
PSRC2 |
Neopestalotiopsis sp. |
14.8 |
– |
– |
– |
– |
– |
– |
– |
– |
14.8 |
PSLC1 |
Nigrospora oryzae (Berk. & Broome) Petch |
– |
– |
11.1 |
– |
– |
– |
– |
– |
– |
11.1 |
|
PSLC2 |
Nodulisporium gregarium (Berk. & M.A. Curtis) J.A. Mey. |
– |
– |
11.1 |
– |
– |
– |
– |
– |
– |
11.1 |
|
PGSC1 |
Daldinia eschscholtzii (Ehrenb.) Rehm |
– |
– |
– |
– |
– |
– |
– |
18.5 |
|
18.5 |
|
PBLC4 |
Pestalotiopsis microspora (Speg.) G.C. Zhao & N. Li |
– |
– |
– |
– |
|
3.7 |
– |
– |
– |
3.7 |
|
Hypocreales
|
PBSC3 |
Fusarium incarnatum complex |
– |
7.4 |
– |
– |
7.4 |
– |
– |
– |
– |
7.4 |
PSSC5 |
Fusarium sp. |
– |
3.7 |
– |
– |
3.7 |
– |
– |
– |
– |
7.4 |
|
PGRC2 |
Fusarium solani complex |
– |
– |
– |
– |
– |
– |
7.4 |
– |
– |
7.4 |
|
MBSC2 |
Simplicillium obclavatum (W. Gams) W. Gams |
– |
– |
– |
– |
11.1 |
– |
– |
– |
– |
11.1 |
|
MBRC1 |
Trichoderma amazonicum complex |
– |
– |
– |
14.8 |
– |
– |
– |
– |
– |
14.8 |
|
Eurotiales |
PBRC2 |
Aspergillus niger complex |
– |
18.5 |
– |
18.5 |
– |
– |
7.4 |
– |
– |
44.4 |
MGSC1 |
Penicillium cataractarum complex |
– |
– |
– |
– |
– |
– |
3.7 |
3.7 |
3.7 |
11.1 |
|
PBRC1 |
Aspergillus flavus complex |
– |
– |
– |
3.7 |
– |
– |
– |
– |
– |
3.7 |
|
Diaporthales |
MBLC2 |
Diaporthe sp. |
– |
– |
11.1 |
– |
– |
11.1 |
– |
– |
3.7 |
25.9 |
PSSC3 |
Diaporthe liquidambaris (C.Q. Chang, Z.D. Jiang & P.K. Chi) Udayanga & Castl. |
3.7 |
– |
– |
– |
– |
– |
– |
– |
– |
3.7 |
|
PSSC7 |
Diaporthe sp. |
|
3.7 |
– |
– |
– |
– |
– |
– |
– |
3.7 |
|
Botryosphaeriales |
MGRC2 |
Macrophomina phaseolina (Tassi) Goid. |
7.4 |
– |
– |
3.7 |
– |
– |
– |
– |
– |
11.1 |
MGLC1
|
Lasiodiplodia citricola Abdollahz., Javadi & A.J.L. Phillips |
|
– |
– |
|
|
– |
– |
– |
11.1 |
11.1 |
|
LT |
Lasiodiplodia theobromae (Pat.) Griffon & Maubl. |
3.7 |
3.7 |
3.7 |
– |
– |
– |
– |
– |
– |
11.1 |
|
Pleosporales |
PSRC3 |
Ectophoma multirostrata (P.N. Mathur, S.K. Menon & Thirum.) Valenz.-Lopez, J.F. Cano, Crous, Guarro & Stchigel |
3.7 |
– |
– |
– |
– |
– |
– |
– |
– |
3.7 |
PSRC4 |
Paraphoma radicina (McAlpine) Morgan-Jones & J.F. White |
7.4 |
7.4 |
– |
– |
– |
– |
– |
– |
– |
11.1 |
|
Glomerellales |
MBLC1 |
Colletotrichum orbiculare complex |
– |
– |
– |
3.7 |
3.7 |
3.7 |
– |
– |
– |
11.1 |
|
PBLC1 |
Non sporulating |
– |
– |
– |
– |
– |
11.1 |
– |
– |
– |
11.1 |
PGRC4 |
Non sporulating |
– |
– |
– |
– |
– |
|
11.1 |
3.7 |
– |
14.8 |
|
PSSC6 |
Non sporulating |
|
3.7 |
– |
– |
– |
– |
– |
– |
– |
3.7 |
|
PSSC2 |
Non sporulating |
– |
3.7 |
– |
– |
– |
– |
– |
– |
– |
3.7 |
|
PSSC1 |
Non sporulating |
– |
14.8 |
– |
– |
– |
– |
– |
– |
– |
14.8 |
Prior studies on endophytic fungi have revealed their affinity for particular tissues, demonstrating how well-adapted they are to tissues 16,34. Tissue base specificity was also observed in root stem and leaf segments of O. tenuiflorum, O. basilicum, and O. gratissimum. Neopestalotiopsis, D.liquidambaris, Ectophoma multirostrata, Trichoderma amazonicum complex, and F.solani complex and A.flavus complex were isolated only from root segments. Diaporthe sp., Simplicillium obclavatum, and Daldinia eschscholtzii and three non – sporulating morphotypes were purely colonized on stem segments, and Nigrospora oryzae, Pestalotiopsis microspora ,Nodulisporium gregarium, Lasiodoplodia citricola and a non -sporulating morphotype were obtained from leaf segments only. Along with the tissue specificity, endophytes showed host preference. F. incarnatum complex and a Fusarium sp. were isolated from stem segments and M. phaseolina was isolated from root segments of O. tenuiflorum and O. basilicum. Penicillium cataractarum complex is obtained only from O.gratissimum, but L. theobromae were isolated from all segments of O. tenuiflorum and C. orbicularae complex from O. basilicum. root and stem segments of O. tenuiflorum yielded P.radicina while O. gratissimum root and stem segments yielded a non-sporulating isolate. Fungi, however, including A. niger complex and Diaporthe sp. (MBLC2), showed occurrence in all host plants. Among the total isolates, S. obclavatum, N. gregarium, P. radicina, D. eschscholtzii, Pestalotiopsis microspora, E. multirostrata, and Trichoderma amazonicum complex were reported new to Ocimum sp., whereas species of Colletotrichum, Penicillium, Aspergillus, Nigrospora, Fusarium, Macrophomina, Diaporthe, and Lasiodiplodia were already reported species from Ocimum plants 35,36,37,16.
Enzyme activity
The extracellular production of amylase, cellulase, laccase, tyrosinase, protease, and asparaginase was screened for all isolated endophytic fungi. Among the 27 isolates, 17 cultures were positive for any of the tested enzymes. Amylase activity was found in 18.5% of the tested isolates. A significant level of amylase activity was found in the isolate Pestalotiopsis microspora from the leaf of O. basilicum and in A. flavus complex and Penicillium cataractarum complex. Previous studies have reported in vitro extracellular amylase activity for Aspergillus spp., Diaporthe spp., Penicillium spp., and Pestalotiopsis microspora 38,39,40. Nine of the tested cultures produced cellulase enzyme. The overall percentage of cellulase activity found was 33.3%. The highest activity was obtained from the fungus S. obclavatum, isolated from the stem segments of O. basilicum. Of all the isolates studied, 7.4% were found to be protease producers. The highest activity was obtained from Penicillium cataractarum complex, which was recovered from the stem segment of O. gratissimum. Studies by Bezerra et al.41 also reported the protease production ability of Penicillium species as an endophyte. Two isolates were positive for tyrosinase. The percentage of positivity was 7.4%. The highest activity was shown by F. solani complex which was isolated from the root segment of O. gratissimum. In the case of asparaginase activity, the colour of the inoculated medium turned pinkish only in 7.4% of the isolates. Asparaginase was positive only for Lasiodiplodia sp. L. citricola showed higher activity than L. theobromae (Table. 3). Due to the fact that fungal asparaginase is produced extracellularly and is a very easy enzyme to purify, it has acquired considerable importance. According to other studies, Alternaria spp., Aspergillus spp., Cylindrocarpon spp., Mucor spp., Fusarium spp., Penicillium spp., and Cladosporium spp. are the most common asparaginase-producing fungi 42,43,44.
Table 3: Enzyme activity of endophytic fungi from Ocimum species
|
Enzyme index |
|||||
Name |
Amylase |
Cellulase |
Protease |
Tyrosinase |
Asparaginase* |
Laccase |
Pestalotiopsis microspora |
1.12 |
– |
– |
– |
– |
– |
Aspergillus flavus complex |
1.06 |
– |
– |
– |
– |
– |
Non sporulating (PSSC1) |
1.09 |
– |
1.06 |
– |
– |
– |
Diaporthe sp. (MBLC2) |
1.07 |
– |
– |
– |
– |
– |
Penicillium cataractarum complex |
1.09 |
– |
1.13 |
– |
– |
– |
Diaporthe sps. (PSSC7) |
– |
1.1 |
– |
1.11 |
– |
– |
Aspergillus niger complex |
– |
1.1 |
– |
– |
– |
– |
Simplicillium obclavatum |
– |
1.16 |
– |
– |
– |
– |
Colletotrichum orbiculare complex |
– |
1.1 |
– |
– |
– |
– |
Macrophomina phaseolina |
– |
1.06 |
– |
– |
– |
– |
Diaporthe liquidambaris |
– |
1.1 |
– |
– |
– |
– |
Paraphoma radicina |
– |
1.06 |
– |
– |
– |
– |
Fusarium sp. (PSSC5) |
– |
1.1 |
– |
– |
– |
– |
Non sporulating (PSSC6) |
– |
1.05 |
– |
– |
– |
– |
Fusarium solani complex |
– |
– |
– |
1.16 |
– |
– |
Lasiodiplodia citricola |
– |
– |
– |
– |
+++ |
– |
Lasiodiplodia theobromae |
– |
– |
– |
– |
++ |
– |
* – for asparaginase production, the colour of the media changes from yellow to pink, +++ indicates the pinkish colour intensity which means they are potentail producer of asparagianse
No enzyme activity was detected at Neopestalotiopsis sp., T. amazonicum complex, D. eschscholtzii, N. oryzae, N. gregarium, F. incarnatum complex, E. multirostrata, A. niger complex and non-sporulating isolates PBLC1, PSSC2 and PGRC4. Laccase enzyme activity was not reported. Previous studies reported the laccase producing ability of the taxon Trichoderma 45, 46,47. It has been reported that Trichoderma harzianum can produce laccase enzyme by using wheat bran under solid state fermentation and by response surface approach48,49. In contrast to previous results, Trichoderma associated with Ocimum spp. does not show any laccase activity.
Based on enzyme activity, five cultures namely S. obclavatum, L. citricola, Penicillium cataractarum complex, Pestalotiopsis microspora and F. solani complex were showed higher activity for cellulase, asparaginase, amylase, protease and tyrosinase respectively were selected and they were characterized molecularly (Table.4)
Table 4: Molecularly identified morphotypes with accession number.
Culture Number |
Identified Name |
Accession Number |
Reference accession number |
Percentage of Similarity |
Reference |
MBSC2 |
Simplicillium obclavatum (W. Gams) W. Gams |
ON344838.1 |
MT487854.1 |
100 % |
Unpublished |
MGLC1 |
Lasiodiplodia citricola Abdollahz., Javadi & A.J.L. Phillips |
ON319006.1 |
MT587428.1 |
100 % |
Zhang et al. 50 |
MGSC1 |
Penicillium cataractarum complex |
ON319003.1 |
MK534497.1 |
99.77 % |
Unpublished |
PBLC4 |
Pestalotiopsis microspora (Speg.) G.C. Zhao & N. Li |
ON342908.1 |
ON796998.1 |
100 % |
Unpublished |
PGRC2 |
Fusarium solani complex |
ON319007.1 |
MT293620.1 |
100 % |
Unpublished |
Conclusion
In this study, 27 endophytic fungi were identified from Ocimum tenuiflorum, O. gratissimum, and O. basilicum, including five non-sporulating. These fungi were able to produce extracellular enzymes such as amylase, cellulase, tyrosinase, protease, and asparaginase. The study emphasizes the importance of fungal endophytes of Ocimum plants and their role in extracellular enzyme production. However, our study is preliminary and by intensive analyzing with different growth media and sterilization protocol, more diversity of endophytic mycobiota of the genus Ocimum may be detected. Advanced studies on enzyme production and quantification are also important to assess the potential significance of these endophytes.
Acknowledgment
The authors are grateful to the University Grand Commission for Junior Research Fellowship for financial support and the Post Graduate & Research Dept. of Botany, Government Victoria College, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram-Kerala, Omics Gen Life Science Pvt. Ltd., and National Fungal Culture Collection of India (NFCCI)- Agharkar Research Institute (ARI) Pune for the lab facility.
Conflict of Interest
The authors declare that, there is no conflict of interest
Funding Sources
This work was supported by university grand commission, new Delhi under junior research fellowship.
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