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Chunduri J. R, Singh P. P, Nair A. P, Patel N. A, Mehta S. B. Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study. Biosci Biotech Res Asia 2023;20(1).
Manuscript received on : 06-11-2022
Manuscript accepted on :  21-01-2023
Published online on:  08-02-2023

Plagiarism Check: Yes

Reviewed by: Dr. Hind Shakir Ahmed

Second Review by: Dr. Vishal Patel

Final Approval by: Dr. Eugene A. Silow

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Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study

Jayaprada Rao Chunduri1* , Prarthana Singh P2, Arati P Nair1, Nidhi A Patel1 and  Sonali B Mehta1

1Department of Biotechnology, SVKM’S Mithibai College of Arts, Chauhan Institute of Science, Amrutben Jivanlal College of Commerce and Economics, Bhaktivedanta Marg, Mumbai India.

2Food Technologist(R and D Executive),Snackible co. ltd., Mumbai-72 India.

Corresponding Author E-mail:jayapradachunduri@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3082

ABSTRACT: Microbes present in the digestive tract of an organism are of great importance. The microbial qualitative and quantitative composition represents the physical, physiological conditions, habits, habitats of the organisms as well as their association patterns. The microbes of digestive system have prominent role not only in the sustainability of the organism but also in the food processing industry. The marine cephalopods are one of the preferred marine food resources, next to marine fisheries. The current study aims to understand the microbial content in the digestive system of consumable cephalopods such as sepia and cuttlefish. The commercially available squids from the market and cuttlefish samples from fishing area were collected and used for this study. The organisms were dissected in aseptic conditions and bacteria collected from the guts of these animals. Culturable bacteria were isolated and, identified using gram characteristics as well as 16s r RNA techniques based molecular identification. The identified bacteria were reported to Gen Bank submission. Bacterial representation in the gut microbiome of cephalopods is low and showed distinct difference between cuttlefish and squid species. This low number of bacterial composition may be due to the habitat conditions or the association of bacteria with the animal requires further studies to understand. The commercial value of cephalopods as protein rich food prioritizes the need to address proper treatment process which can alleviate their presence in Indian food industry scenario. The inter and intra-species relation of microbes and metazoans and the associated macromolecules can be used for pharma industry also in future.

KEYWORDS: Commercial value; Cephalopod; Digestive tract; Microbiome; 16 S ribosomal RNA

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Chunduri J. R, Singh P. P, Nair A. P, Patel N. A, Mehta S. B. Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study. Biosci Biotech Res Asia 2023;20(1).
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Chunduri J. R, Singh P. P, Nair A. P, Patel N. A, Mehta S. B. Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study. Biosci Biotech Res Asia 2023;20(1). Available from: https://bit.ly/3HMVIaf

Introduction

Cephalopods are potential alternate protein rich food and have significant place in export commerce. They emerge as important contributors to the need of the country and future resources. They should be constantly monitored as they play a prominent role in trophic pyramid1. Cephalopods require constant monitoring of their stock as they became a matter of concern due to climatic change or environmental disturbances 2-4.  The major catch of the squid species found to be higher (97%) from Indian waters alone, followed by Indo-Pacific Ocean periphery, Pink sea, Arabian sea, Mozambique to South China Sea, Philippines sea and Northern Taiwan. Cornea of the eyes has major contribution while distinguishing the species of   cephalopods. Many studies have been conducted to study the biology, population dynamics, commercial exploitation, feeding habits and the energetic lifestyle of squids. Squid species are cannibalistic and big scale migrations occur during the scarcity of food 5-7. The mass migration of squids permits them to take advantage of spatial and temporal marine production systems equipped with adaptations in the body structure viz. tentacles. They behave as ecological opportunists. Squid meat has an extraordinary nutritive value with high protein bioactive substances and omega 3 and 6 rich marine oil content which makes them most appropriate for human consumption 8,9. Processing methods such as canning and drying followed by freezing are done prior to their sale in the domestic or overseas markets. The previous studies of cephalopods emphasized the ecology, catch, environmental impacts and food and feeding habits at national and international level 10-13. Earlier studies on Sepia officinalis, Octopus sps., Loligo vulgaris and Nautilus popilius mainly focused on the cephalopod’s digestive system physiology or morphology, motility and absorptive functions up to a limited extent 14.  Analyses of the cephalopod gut microbiome are limited in spite of their key phylogenetic position, ecological and commercial importance 15.  A unique set of microbial composition of the gut microbiota was observed in the study of six cephalopod species in comparison to their marine mollusk and fish counterparts16.  Further, the gut microbiome of aquarium cultured cuttlefish indicated symbiotic relationship with that of the host 17. The current study aims to assess the composition of digestive tract microbiome in selected cephalopods off India using the culture methods.  In addition, the possible reasons for their unique pattern of representation in the cephalopods and their importance in the food industry were analyzed.

Material and methods

Isolation of bacteria from Squid and cuttlefish

Squid and cuttlefish samples were procured from the commercial and fishing markets and transferred to the laboratory.  Standard FAO catalogues of Cephalopods were used for the identification of these organisms 18,19. The organisms were surface sterilized by washing with water, wipe them with absolute alcohol and 30 sec. immersion in the alcohol. The digestive tract was removed and placed in 10 ml of sterile saline solution after dissecting the organisms under aseptic conditions.  The tract was teased and the contents were emptied with 5ml of sterile saline.  1ml of aliquot of gut homogenate was serially diluted with sterile saline up to 10-7 dilution.  This 0.1 ml of homogenate was spread on Nutrient and Cetrimide agar media and incubated at 37°C for 24 hrs. Gram staining was done morphological characterization to distinguish between the Gram positive/Gram negative  as well the shape of individual organisms. The florescence was checked under UV. The selected bacterial isolates from squid and cuttlefish were further transferred on Nutrient agar slants and sent for 16S rRNA based identification to Credora Life Sciences, Bangalore.

Molecular characterization of cephalopod gut associated bacteria

Identification of bacteria based on16S r RNA Sequencing

Hyper variable regions within the 16S r RNA gene represent a species unique signature used for bacterial identification. The CTAB method (N-cetyl, NNN Trimethyl Ammonium Bromide) was employed for the extraction of bacterial genomic DNA.  The obtained DNA pellet was washed with 70% Ethanol, and dissolved in 20 µl sterile distilled water. The genomic DNA was read at wavelengths of 260oA and 280oA using UV-VIS spectrophotometer (Viva spec Bio -Spectrophotometer, Germany) for establishing purity and quantification.

The amplification of 16S region was performed using Polymerase Chain Reaction technique. A reaction mixture of 20 µl comprising- Polymerase Chain Reaction buffer (PCR buffer) , ddNTP, dNTP mixture, Taq polymerase, primer, template DNA was used. Sterile nuclease free water was considered as negative control. PCR temperature profile comprised a denaturation stage (2 min 50 sec at 94°C),  Annealing stage (30s  at 48°C),  Extension stage (1min 30 sec. at 72°C) followed by final extension stage of 6 min. Oligonucleotide primers of 500bp with a length of 20 nucleotides  were considered as 16 S forward primer of (5′-3′)  AGAGTTTGATCMTGGCTCAG, 50% GC and Tm Value 51.0°C and, 16S Reverse Primer Sequence (5’-3’) of CGGTTACCTTGTTACGACTT with 60 % GC, Tm Value 56.0°C 20  were  considered for the study. A comparison of 16S r RNA sequence of bacterial genome was carried out based on NCBI BLAST and bacterial phylogenetic status by multiple sequence alignment (CLUSTAL-O 1.2.4) . The closely related organism was identified and the sequences were deposited to the NCBI Gen Bank to obtain accession numbers.

Extraction and identification of proteins

A loopful  of  fluorescent bacteria (isolated from the squid) inoculum was added to 50ml of Nutrient broth. The culture medium was collected after 48 hrs of incubation at 370 C on shaker ( 500 rpm) conditions and  centrifuged. Proteins were separated using salting and desalting techniques and further purified using dialysis from the spent medium 21. The proteins were separated using Native PAGE.  The protein band was further analyzed using Orbitrap HRLC-MS technique (Thermo EASY-Nlc and Q Exactive Plus – Orbitrap MS) at SAIF, IIT, Mumbai.

Results and Discussion

Cephalopods are active marine predators. The earlier studies on their digestive system indicated many physiological adaptations in accordance with their habit and habitat.  The physiology of digestive tract was monitored using non-invasive techniques.  The welfare of the cephalopod in aquaculture and their digestive system shown functional relevance 22,23. However, less priority was given to the studies aimed at contents of digestive tract of a cephalopods which play a major role in laboratory maintenance of animals as well as aquaculture practices14 . A comparative study of guts of 6 cephalopod species and other mollusks, with that of marine fish was carried out to investigate the factors that shape the gut microbiota15,16.

Isolation of bacteria from Squid and cuttlefish

The results of current microbial contents of the gut based on culture based studies indicated the presence of gram-negative bacteria rods with green florescence under UV 365nm. The bacteria from squid showed growth in Cetrimide agar. Qualitatively, only two different bacterial variants were observed in the gut of squid, and 4 different bacterial variants from the gut of cuttlefish during the study.

Molecular characterization of cephalopod gut associated bacteria

The 16S r RNA amplicon BLAST sequence similarity with bacterial databases identified Proteobacterial species.  The sequence based similarity studies using BLAST indicated the variants were closely related to Alcaligenes and Stenotrophomonas species (93.65% and 86.46% respectively) represented Class Betaprotebacteria and Gammaproteobacteria. The bacterial species’ nucleotide sequences were submitted to NCBI (Accession no. s – ON 524799 and ON 52498). The Gen Bank Accession no. ON524798 represented the Stenotrophomonas sp. strain jpm20  16S ribosomal RNA gene, partial sequence   of 948bp.   Similarly, the second isolate sequence submitted  was given Gen Bank Accession no. ON 524799 represented  as Alcaligenes sp. strain Sloli A09 16S ribosomal RNA gene partial sequence (Fig.1). Earlier studies on squid indicated the presence of Photobacterium sps. a bioluminescent bacterium 24.  Of the above two species Stenotrophomonas has been identified as a bioluminescent bacterium which produced a florescent protein in the medium during culturing.

Figure 1: Phylogenetic associations of Stenotrophomonas sp. Strain jpm 20 (a) , Alcaligenes sp. Strain sloli A09

Click here to view figure

Previous studies on laboratory maintained aquarium cuttlefish (Sepia officinalis) at Marine Biological Laboratory, Woods Hole indicated the presence of symbiotic bacteria in the gut of these animals. The simple microbiome of two bacteria amplicon variants (ASVs) represented the entire digestive tract 18 and belonged to Vibrionaceae and Piscirickettsiaceae of Gammaproteobacteria. During the current study, 4 isolates represented the entire digestive tract of cuttlefish were identified by  the 16S r RNA amplicon based BLAST similarity (>83%) search  revealed bacteria  belonged to Flavobacteria, Gammaproteobacteria, and Betaproteobacteria classes of Proteobacteria.  The 16s R RNA genes of bacterial variants  indicated  a  BLAST similarity of variant 1 with Myroides odoratimimus strain pgdne3 (93.09 %);  Variant 2 with  Pseudomonas putida strain BPA1 (92.61%) ;  variant 3  with  >85%  to an Uncultured bacterium clone Shelves_B_90  and, variant 4  showed with Pseudomonas viridiflava strain CE9 (88.35 % ).  The representative 16S r RNA gene sequences of the variants were submitted to Gen Bank. The  Gene bank  accession numbers  ON 838558, ON838559, ON 838560  and ON624237 were assigned  partial 16s RNA genes of  to Pseudomonas fluorescens (664 bp); Burkholderiales bacterium  (788 bp);  Syntrophobacterales bacterium (217bp ) and, Pseudomonas sp. strain ansj 3 (707bp ). 

Extraction and identification of proteins

The Liquid Chromatography-Mass Spectrometry (LC-MS) analysis indicated a spectrum of total 240 proteins isolated by protein analysis (Table-1). The heat map indicated the accession number of proteins extracted, number of peptides, overall molecular weight and number of amino acid present.  Of which overall molecular weight and number of amino acid present showed variations. Among these, 29 unidentified proteins were further identified and analyzed using the UNIPROT and PDB Data bases and BLAST.       

The study indicated few of the unidentified proteins represented Type IV secretion systems’ macromolecules. The proteins of T4SS are characterized as type IV secretion protein which can be utilized for inter-bacterial killing. Earlier studies of Stenotrophomonas sps expressed proficiency in killing competitor bacterial species especially Escherichia coli, Klebsiella pneumonia, Salmonella typhii and Pseudomonas aeruginosa (gram –ve)25.  The invasive proteins (A0A4Z0DSX7), a metallic protein similar to that of an immunity protein (PDB id.6PDK) along with type IV secretion system protein virB5 Extraction and identification of proteins (A0A0U5I2Y5) were some of the proteins encountered based on the LC_MS based analysis.  The VirB2 and VirB5 proteins mediate host-cell targeting.  They behave like adhesins and, initiate target binding to specific host receptors 26.  The above reason can be a possible reasonable explanation for the unique representation of only 2 species of bacteria in the squid digestive tract.

Research analysis of gut microbiota of six species of wild cephalopods by Illumina Mi Seq sequencing of 16S r RNA gene amplicons indicated that each cephalopod gut consisted of a distinct consortium of microbes. The gut microbial composition reflects host phylogeny15,16.   Previous studies indicated that healthy octopuses and squids are associated mainly with Vibrio sps followed by members of other genera such as Pseudomonas, Aeromonas, Staphylococcus or Streptococcus sps. 27. The presence of Stenotrophomonas sp., Alcaligenes sps, Pseudomonas sps, and Myroides sps require detailed study to assess   their role  as opportunistic pathogens that can affect human life or probable probiotics, or in bioremediation, production of antibiotics or other industrial uses28,29.

The presence of florescent bacteria offers protection and of symbiotic  relation which can be seen in benthic organisms such as squid and cuttlefish. The current study emphasized the observation of 2 species in squids and 4 species in cuttlefish could be due to their phylogenetic characteristics.  A careful processing of these cephalopods is necessary as the external microbial contamination can be removed as well as, the internal microbial population can be source of food spoilage as well as food poisoning.

Table 1: Heat map of Stentrophomonas sps. secreted Proteins extracted and characterized using LC-MS

Acession No

Coverage [%]

#  Peptides/uniqueseq/PSMS

# AAs

MW [kDa]

calc. pI

A0A2R7P3G6

–

–

–

–

–

A0A3N1K3U7

–

–

–

–

–

A0A3D0GD54

–

–

–

–

–

A0A4Z0DW87

–

–

–

–

–

A0A1W5DQU1

–

–

–

–

–

A0A1W5DRI2

–

–

–

–

–

A0A4V2FEE5

–

–

–

–

–

A0A1B2N6P9

–

–

–

–

–

A0A317BNT7

–

–

–

–

–

A0A239QDD8

–

–

–

–

–

A0A4V1WIG2

–

–

–

–

–

A0A1W5DR29

–

–

–

–

–

A0A0J8PQL4

–

–

–

–

–

A0A1W5DFW2

–

–

–

–

–

A0A1Y5Q6G1

–

–

–

–

–

A0A0Q4QD17

–

–

–

–

–

A0A4U9PMS0

–

–

–

–

–

A0A4Z0DRP5

–

–

–

–

–

A0A0F5ZPL3

–

–

–

–

–

A0A2T3WJN2

–

–

–

–

–

A0A1W5DIJ8

–

–

–

–

–

A0A1W5DU88

–

–

–

–

–

A0A0F5ZPP7

–

–

–

–

–

A0A1W5DRY1

–

–

–

–

–

A0A380AS26

–

–

–

–

–

A0A2W5KX19

–

–

–

–

–

A0A1W5DSI9

–

–

–

–

–

A0A3C0X491

–

–

–

–

–

A0A2T1JG43

–

–

–

–

–

A0A1W5DRR9

–

–

–

–

–

A0A4Z0DS73

–

–

–

–

–

A0A4Z0E2Z9

–

–

–

–

–

A0A355YHK4

–

–

–

–

–

A0A2D0AP22

–

–

–

–

–

A0A0R0E5D6

–

–

–

–

–

A0A1W5DS87

–

–

–

–

–

A0A4Z0DQS9

–

–

–

–

–

A0A2W5NZ56

–

–

–

–

–

A0A1B2N8H6

–

–

–

–

–

A0A4Q7R830

–

–

–

–

–

A0A1W5DS28

–

–

–

–

–

A0A0R0ALV5

–

–

–

–

–

A0A1W5DSR5

–

–

–

–

–

A0A4Z0DZN7

–

–

–

–

–

A0A1W5DHR6

–

–

–

–

–

A0A4S2D5F6

–

–

–

–

–

A0A2W5NWF3

–

–

–

–

–

A0A1W5DSJ9

–

–

–

–

–

A0A0R0CY49

–

–

–

–

–

A0A431UAC2

–

–

–

–

–

A0A4Z0DQY0

–

–

–

–

–

A0A4Q4LAS4

–

–

–

–

–

A0A498CIE5

–

–

–

–

–

A0A023Y629

–

–

–

–

–

A0A0R0ANU2

–

–

–

–

–

A0A1W5DSQ8

–

–

–

–

–

A0A1W5DRL3

–

–

–

–

–

A0A4U3GQA6

–

–

–

–

–

A0A1D8YAH9

–

–

–

–

–

A0A4Z0E388

–

–

–

–

–

A0A3C0XAW0

–

–

–

–

–

A0A4Z0DSX7

–

–

–

–

–

A0A1Q4ESR5

–

–

–

–

–

A0A1B2N4G9

–

–

–

–

–

A0A3D0GES6

–

–

–

–

–

A0A0R0C9X0

–

–

–

–

–

A0A1W5DS47

–

–

–

–

–

A0A023XZR8

–

–

–

–

–

A0A4Z0DV97

–

–

–

–

–

A0A2J0SNC4

–

–

–

–

–

A0A2W5LFJ7

–

–

–

–

–

A0A1W5DGG2

–

–

–

–

–

A0A1W5DPF7

–

–

–

–

–

A0A4Z0E308

–

–

–

–

–

A0A4Z0DRP6

–

–

–

–

–

A0A5N7SB45

–

–

–

–

–

A0A4Z0DT03

–

–

–

–

–

A0A2Y9UCH2

–

–

–

–

–

A0A0U5I2Y5

–

–

–

–

–

A0A357N7P7

–

–

–

–

–

A0A4Z0DRX0

–

–

–

–

–

A0A246HIT6

–

–

–

–

–

A0A3S7KNA5

–

–

–

–

–

A0A4Z0E039

–

–

–

–

–

A0A4Z0DTP5

–

–

–

–

–

A0A0M1EJ90

–

–

–

–

–

A0A4Z0E332

–

–

–

–

–

A0A4Q1CX40

–

–

–

–

–

A0A3D0GCI2

–

–

–

–

–

A0A4Q4LIK9

–

–

–

–

–

A0A2W5LZC2

–

–

–

–

–

A0A2W5LSV3

–

–

–

–

–

A0A246HJA3

–

–

–

–

–

A0A2T1IS34

–

–

–

–

–

A0A4Z0DZ24

–

–

–

–

–

A0A0R0C9W0

–

–

–

–

–

A0A431U9V6

–

–

–

–

–

A0A4Z0DU32

–

–

–

–

–

A0A4Z0DXY9

–

–

–

–

–

A0A0R0CYG6

–

–

–

–

–

A0A355YD66

–

–

–

–

–

A0A4Z0E1X7

–

–

–

–

–

A0A355YHR5

–

–

–

–

–

A0A0R0BF08

–

–

–

–

–

A0A0R0DYW2

–

–

–

–

–

A0A4V2FEB3

–

–

–

–

–

A0A1D8YA83

–

–

–

–

–

A0A4Z0DUQ4

–

–

–

–

–

A0A1W5DRJ6

–

–

–

–

–

A0A4Z0E493

–

–

–

–

–

A0A0R0DWS3

–

–

–

–

–

A0A4Z0DZF0

–

–

–

–

–

A0A397M9Y6

–

–

–

–

–

A0A0R0DZT9

–

–

–

–

–

M5D7E1

–

–

–

–

–

A0A4Z0E1N0

–

–

–

–

–

A0A1B2N4U5

–

–

–

–

–

A0A4Z0E0Y5

–

–

–

–

–

A0A4Q7RU54

–

–

–

–

–

A0A3D4TK95

–

–

–

–

–

A0A0R0CFM5

–

–

–

–

–

A0A4Z0DYQ0

–

–

–

–

–

A0A498CHN1

–

–

–

–

–

B8LAU6

–

–

–

–

–

A0A4Z0DRJ7

–

–

–

–

–

A0A4Q4LE00

–

–

–

–

–

A0A0R0CKP9

–

–

–

–

–

A0A3N1R039

–

–

–

–

–

B4SIW5

–

–

–

–

–

A0A0R0AIG5

–

–

–

–

–

A0A0R0CMQ8

–

–

–

–

–

A0A2R7QQI7

–

–

–

–

–

A0A4Z0DRF1

–

–

–

–

–

A0A4Z0DZL8

–

–

–

–

–

A0A3D5JS11

–

–

–

–

–

A0A4Z0DR01

–

–

–

–

–

A0A0M0P1V9

–

–

–

–

–

A0A4Z0DWL6

–

–

–

–

–

A0A2T3X2F7

–

–

–

–

–

A0A0R0E4I2

–

–

–

–

–

A0A4R4IC51

–

–

–

–

–

A0A0R0D8D1

–

–

–

–

–

Q7X0G1

–

–

–

–

–

A0A4Z0DZF3

–

–

–

–

–

A0A0R0DIQ7

–

–

–

–

–

A0A3N1K6Q6

–

–

–

–

–

A0A4Z0DZV7

–

–

–

–

–

A0A4Z0E1M1

–

–

–

–

–

A0A1D8Y7J2

–

–

–

–

–

A0A2J0UGT4

–

–

–

–

–

A0A0R0BSW6

–

–

–

–

–

A0A4T1DKR3

–

–

–

–

–

A0A246HIC6

–

–

–

–

–

A0A0R0CXC6

–

–

–

–

–

A0A1W5DGS1

–

–

–

–

–

A0A0R0DHF7

–

–

–

–

–

A0A0U5HY80

–

–

–

–

–

A0A0R0D0D2

–

–

–

–

–

A0A0R0CHQ6

–

–

–

–

–

A0A5N0KMT0

–

–

–

–

–

A0A4Z0E174

–

–

–

–

–

A0A0R0C2W5

–

–

–

–

–

A0A4Q1CRR0

–

–

–

–

–

A0A1Q4EXN0

–

–

–

–

–

A0A5N0KRC7

–

–

–

–

–

A0A353U758

–

–

–

–

–

A0A1W5DSD8

–

–

–

–

–

A0A4Z0E2D3

–

–

–

–

–

A0A0Q4QUV2

–

–

–

–

–

A0A2J0UAU1

–

–

–

–

–

A0A4Z0DY99

–

–

–

–

–

A0A0R0AFI0

–

–

–

–

–

A0A0K2IF90

–

–

–

–

–

A0A0Q4QGX2

–

–

–

–

–

A0A0K2IUH4

–

–

–

–

–

A0A4Z0DZE2

–

–

–

–

–

A0A0Q4QF94

–

–

–

–

–

A0A4Z0DQM8

–

–

–

–

–

A0A498C240

–

–

–

–

–

A0A1D8YEE6

–

–

–

–

–

A0A4Z0E2G8

–

–

–

–

–

A0A0R0CSI7

–

–

–

–

–

A0A0R0CHY8

–

–

–

–

–

A0A4Z0DSG8

–

–

–

–

–

A0A4Z0E1S1

–

–

–

–

–

A0A2Y9U3Z2

–

–

–

–

–

A0A4Z0E0I4

–

–

–

–

–

A0A4Z0E192

–

–

–

–

–

A0A4Z0DYN4

–

–

–

–

–

A0A2T1IP77

–

–

–

–

–

A0A2M9QZ64

–

–

–

–

–

A0A4Z0E0R4

–

–

–

–

–

A0A0R0CV94

–

–

–

–

–

A0A2J0UX67

–

–

–

–

–

A0A4V2FGS0

–

–

–

–

–

A0A2M9QX77

–

–

–

–

–

A0A4Z0E095

–

–

–

–

–

A0A1D8Y994

–

–

–

–

–

A0A4Z0E1V8

–

–

–

–

–

A0A2D2W2A6

–

–

–

–

–

A0A0R0AAH8

–

–

–

–

–

T5KP48

–

–

–

–

–

A0A4R7US30

–

–

–

–

–

A0A4Z0DQY3

–

–

–

–

–

A0A4Z0DYU7

–

–

–

–

–

A0A1A6Y0L8

–

–

–

–

–

A0A4Z0E1H0

–

–

–

–

–

A0A4Z0DQK3

–

–

–

–

–

A0A023Y783

–

–

–

–

–

A0A0M1EV12

–

–

–

–

–

A0A149Q5G4

–

–

–

–

–

A0A3N1KP13

–

–

–

–

–

A0A363S4I9

–

–

–

–

–

A0A2J0T2Q8

–

–

–

–

–

A0A0D0JM52

–

–

–

–

–

A0A0R0BJ61

–

–

–

–

–

A0A4Z0E112

–

–

–

–

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A0A4Z0E327

–

–

–

–

–

A0A2W5LHA7

–

–

–

–

–

A0A4Z0DYA1

–

–

–

–

–

A0A427CF66

–

–

–

–

–

A0A2U4HFE5

–

–

–

–

–

A0A0R0AMU9

–

–

–

–

–

A0A4Z0E3J7

–

–

–

–

–

A0A2W6G348

–

–

–

–

–

A0A2R7R082

–

–

–

–

–

A0A3N1QLM7

–

–

–

–

–

A0A0R0CQ61

–

–

–

–

–

A0A4Z0DXA0

–

–

–

–

–

A0A4Z0DR66

–

–

–

–

–

A0A2M8VN75

–

–

–

–

–

A0A0M0P3Z8

–

–

–

–

–

A0A0U5AYD8

–

–

–

–

–

A0A2W6UQ55

–

–

–

–

–

A0A0R0ARZ9

–

–

–

–

–

A0A4Z0DXN9

–

–

–

–

–

A0A149QFI1

–

–

–

–

–

A0A2J0SNK9

–

–

–

–

–

A0A0R0CUE4

–

–

–

–

–

A0A5K1NT45

–

–

–

–

–

A0A4Z0E2X8

–

–

–

–

–

A0A4Z0E2D4

–

–

–

–

–

A0A0R0E0H4

–

–

–

–

–

A0A0R0BRS4

–

–

–

–

–

A0A2W6HCD0

–

–

–

–

–

A0A3S5GHZ1

–

–

–

–

–

Conclusion

The comparative study of composition of gut microbes from squid and cuttlefish confirmed that the composition of microbes was different in both the cephalopod species of benthic habitat.  The distinct change supports that the pattern follows the phylogeny.   The secreted proteins that are associated with Stenotrophomonas sps. control the other selected bacterial populations indicating the possible reason for the low representation of other species.  These macromolecules that express bactericidal activity can be further used in the drug development studies as well.    Since the squids are delicacy, the qualitative and quantitative bacterial load should be assessed and, warrants for proper treatment process prior to their use as food.

Acknowledgement

The authors thank SVKM Management and I/C Principal Dr. Krutika Desai for the facilities provided. A special mention in appreciation of extended technical co-operation from Credora Life Sciences  and SAIF of IIT Mumbai during the study.

Conflict of Interest

There is no conflict of interest.

Funding Source

There are no funding sources.

References

  1. Anusha, R. and T.F.Albin : Cephalopod: Squid Biology, Ecology and Fisheries in Indian waters- Int. j. fish. aquat. Stud.2014,1(4): 41-50 .
  2. Meiyappan, M. M.and K.S.Mohamed:  In: Status of Exploited Marine Fishery Resources of India. CMFRI, Cochin, (2003pp. 221-227.
  3. Rodhouse,P.G K: Effects of environmental variability and change on cephalopod populations: An introduction to the CIAS ’09 Symposium special issue- ICES J Mar ,2010 67(7):1311-1313.
    CrossRef
  4. Pecl, G.T. and G.D.Jackson: The potential impacts of climate change on inshore squid: biology, ecology and fisheries-Rev Fish Biol Fish, 2008: 18:373-385
    CrossRef
  5. Ibanez, C.M. andKeyl : Cannibalism in cephalopods-Rev Fish Biol Fish., 2011; 20:123-136.
    CrossRef
  6. Roger,V., P.Valentina and Graziano: Cephalopods as Predators: A Short Journey among Behavioral Flexibilities, Adaptions, and Feeding Habits-Front Physiol, 2017;8: 598.
    CrossRef
  7. Trueblood,L.A. and A.Seibel: The jumbo squid, Dosidicus gigas (Ommastrephidae), living in oxygen minimum zones I. Oxygen consumption rates and critical oxygen partial pressures-Deep Sea Research Part II: Topical Studies in Oceanography 2013 ;95:,  218-224
    CrossRef
  8. Asadpour,L.A: Squid (Loligo loligo): The new source to extract omega-3 and omega-6 rich marine oils– J. Fish. Sci. 2016; 15(1):100-107
  9. Rajasekharan, N.J., P.Devika, M.J.Sophia ,P.Gomathi, V.S.Priya and P.V.Sherief: Cephalopod research and bioactive substances. Indian J of Geo-Marine Sciences; 2011:40:13-27.
  10. Nirmala,S.K, S.K.Chakraborty, AKJaiswar, R.P.Swamy, R.Rajapradsad, S.Boomireddy and Rizvi: Growth and mortality of Indian squid, Loligo duvauceli (d’Orbigny) (Mollusca/Cephalopoda/Teuthoidea) from Mumbai waters, India –Indian J of Marine , 2003;32(1):67-70.
  11. Gretta, T.P. andJ.George: The potential impacts of climate changes on inshore squid: biology, ecology and Fisheries Rev. Fish Biol. Fish., 2008; 18:373-385.
    CrossRef
  12. Hanlon, R.T., K.Buresch, D.Staudinger, and H.Moustahfid: Doryteuthis pealeii, Longfin inshore squid. In: Advances in Squid Biology, Ecology, and Fisheries. 2013 (Eds: Rosa, R., Pierce, G., and R. O’Dor) In Press. Nova Science Publishers, Inc. Hauppauge, NY
  13. Luckhurst, E: A preliminary assessment of the ecological role and importance of squid in the pelagictrophic web of the northwest Atlantic Ocean including the Sargasso Sea. ICCAT, 2018;74(7): 3679-3691
  14. Sykes, A.V., E.Almansa, G.M.Cooke, G.Ponte .and L.R.A.Paul The Digestive Tract of Cephalopods: a Neglected Topic of Relevance to Animal Welfare in the Laboratory and Aquaculture. Front Physiol. 2017; 8: 492.
    CrossRef
  15. Kang, W., R.S.Kim, E.J.Tak, H.Sung, N.R.Shin, D.W.Hyun, T.W. Whon, H.S.Kim, J.Y.Lee, J.H.Yun, M.J.Jung, J.W. Bae: Host phylogeny, habitat, and diet are main drivers of the cephalopod and mollusk gut microbiome –Anim Microbiome;. 2022 8;4(1):30.
    CrossRef
  16. Kang, W., P.S.Kim, E.J.Tak, H.Sung,and N.R.Shin: Host Phylogeny Determines The Gut Microbial Landscape of Cephalopods.(Preprint version 2) Research Square .2021. [https://doi.org/10.21203/rs.3.rs-556214/v2]CrossRef
  17. Holly, L.L.S., P.R.Tabita, A.Lisa, D.Amber, S.Cathleen,G.Neil, K.S.Alexandra, T.H.Roger, A.G.Jack, L.M. Jessica, and M.Welch: A Simple Microbiome in the European Common Cuttlefish, Sepia officinalis – mSystems. 2019;4 (4) 00177-19
    CrossRef
  18. Roper, C.F.E., M.J.Sweeney, and C.E.Nauen: FAO species Cephalopods of the world. An annotated and illustrated catalogue of species of interest to fisheries-FAO Fisheries Synopsis.1984,,125(3):277 .
  19. Jereb, FC., E.Roper and M.Vecchione : Cephalopods of the world. An annotated and illustrated catalogue of species known to date. Myopsid and Oegopsid Squids.vol.2
    (Eds.P.C.Jereb,F.E.Roper,and M.Vecchione),.FAO Species Catalogue for Fishery Purposes. Rome2010.35–37.
  20. Sambrook, J. and D.Russell: Molecular Cloning: A Laboratory Manual (3rd ). Cold Spring Harbor Laboratory Press. 2001.  
  21. I.M. (), Protein analysis and purification Benchtop techniques 2nd Edition. Birkhauser. Boston. 2004 ISBN 0-8176-4341-9
  22. Ponte,G.,  A.V.Sykes, G.M.Cooke, E.Almansa, and  L.R.A.Paul:  The Digestive Tract of Cephalopods: Toward Non-invasive In vivo Monitoring of Its Physiology Front Physiol. ; 2017; 8: 403.
    CrossRef
  23. Ponte,G, Almansa and P.L.R.Andrew- Editorial: The Digestive Tract of Cephalopods: At the Interface Between Physiology and Ecology. Front. Physiol. 2018;9:1409.
    CrossRef
  24. Yaser, N.A., M.F.F.Abdullah, A.F. Aris, and I.Zainudin: Isolation And Identification Of Bioluminescent Bacteria In Squid And Water Of Malaysia Int’l Journal of Advances in Agricultural & Environmental Engg.2014; 1 (2.)
    CrossRef
  25. Bayer-Santos, E., Cenens, W., Matsuyama, B.Y., Oka, G.U., Di Sessa, G., Mininel, I.D.V., Alves, T.L., Farah, C.S.The opportunistic pathogen Stenotrophomonas maltophilia utilizes a type IV secretion system for interbacterial killing. PLoS Pathog. 2019 ;15(9): e1007651-e1007651
    CrossRef
  26. Steffen B, Remi F, and Gabriel W. VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems? Trends Microbiol . 2008 ;16(9):409-13
    CrossRef
  27. Farto, R., G.Fichi,Gestal, S.Pascual and T.P.Nieto: Bacteria-Affecting Cephalopods. In: Handbook of Pathogens and Diseases in Cephalopods. (Eds.:C.Gestal, S.Pascual, A.Guerra, G.Fiorito, and J.Vieites.)  Springer, Cham. 2019; 127–142.
    CrossRef
  28. .Marinho, P.R., A.P.Moreira, F.L.Pellegrino, G.Muricy, M.Bastos, K.R.Santos, M.Giambiagi-deMarval and M.S.Laport: Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria. Memorias do Instituto Oswaldo Cruz, 2009; 104(5), 678–682.
    CrossRef
  29. Chinnarajan, R.,V.Govindaswamy, R.Rajasabapathy, V.Logeshwaran, and R.A.Sreepada: Infection and pathogenecity of Myroides odoratimimus (NIOCR-12) isolated from the gut of grey mullet (Mugil cephalus (Linnaeus, 1758))- Microb Pathog., 2015; 88; 22-28
    CrossRef
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