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Published online on: 11-01-2016
Investigation in to the Cell Wall Bound Beta-glucosidase of Penicillium purpurogenum
A. B. Dhake and M. B. Patil*
University Department of Biochemistry, L.I.T. Premises, Nagpur University, Nagpur - 440 033 (India)
ABSTRACT: Penicillium purpurogenum was found to produce cell wall bound b-glucosidase reaching to its peak after 4 days of incubation, which parallels with the growth of fungus. The cell wall bound enzyme exhibited optimum activity at pH 5.5 & 50°C. The ionic detergent SDS at 0.1% concentration shows maximum solubilization of cell wall bound enzyme in to the extraction buffer. The enzyme activity was mildly enhanced by metal ions like K+, Na2+ and strongly inhibited by Hg2+. EDTA shows little inhibition of the b-glucosidase activity.
KEYWORDS: b-glucosidase; SDS; Penicillium purpurogenum
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| Copy the following to cite this article: Dhake A. B, Patil M. B. Investigation in to the Cell Wall Bound Beta-glucosidase of Penicillium purpurogenum. Biosci Biotechnol Res Asia 2005;3(1) |
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