Manuscript accepted on :
Published online on: 28-01-2016
A. G. Namdeo1*, D. P. Fulzele2, S. Patil3 and S.S. Kadam1
1Department of Pharmacognosy and Phyto-Biotechnology, Poona College of Pharmacy, BVDU, Erandwane Educational Campus, Pune (India). 2 Biotechnology Division, Bhabha Atomic Research Centre, Mumbai (India). 3 School of Life Sciences, DAVV, Indore (India)
ABSTRACT: Sucrose is a major ingredient of culture medium. Present study was performed to investigate the influence of sucrose concentration on growth and ajmalicine production. Maximum packed cell volume (72%), fresh weight (65g 100 ml-1), and dry weight (6.2148g 100 ml-1) was recorded in medium containing 4% sucrose. About 9-fold increase in dry weight was recorded in medium with 4% sucrose. All growth parameters increased upto 25th day of incubation after which a gradual decline was observed. Ajmalicine accumulation started from 15th day of incubation and reached to maximum (60 μg g-1 dry weight) in 25th day of incubation in medium with 4% sucrose, which however gradually reduced on further incubation.
KEYWORDS: Catharanthus roseus; sucrose; ajmalicine; suspension culture
Download this article as:
| Copy the following to cite this article: Namdeo A. G, Fulzele D. P, Patil S, Kadam S. S. Influence Of Sucrose Concentration On Growth And Ajmalicine Production From Catharanthus Roseus Suspension Cultures. Biosci Biotechnol Res Asia 2005;3(2) |
| Copy the following to cite this URL: Namdeo A. G, Fulzele D. P, Patil S, Kadam S. S. Influence Of Sucrose Concentration On Growth And Ajmalicine Production From Catharanthus Roseus Suspension Cultures. Biosci Biotechnol Res Asia 2005;3(2). Available from: https://www.biotech-asia.org/?p=5145 |
This work is licensed under a Creative Commons Attribution 4.0 International License.
