Study of the effect of the green tea on the crystallization of brushite in the artificial urine with pH 6.5

Mokhbi Abdelwahab¹ and Bahri Fouad²

¹Departement of Agronomy, Faculty of Science and Sciences of Engineers (Algeria) ²Department of Biology, Faculty of Science and Sciences of Engineers (Algeria)

ABSTRACT: The tea plant, Camellia sinensis is cultivated for its sheets whose infusion and decoction allow the preparation of drink the most consumed in the world. Today, it is almost regarded as a drug. Certain crystalline species are evocative of a urinary supersaturation being able to rise of very different causes. Thus the brushite of the IVd type is induced by hypercalciuries with generally hyperphosphaturie and /or hyperparathyro?die, disorder of the acidification, renal anatomical anomaly. The description of a possible inhibiting action of the tea on phosphocalcic crystallization with pH=6.5 can have very positive repercussions on the public health. That encouraged us to test in vitro the effect of its infusion and its decoction with three different concentrations on phosphocalcic crystallization starting from the artificial urine with pH=6.5. In this objective, we thus developed a simple method of microphotography of which the duration of the experiment is 41 minutes. It makes it possible to appreciate the three phases of crystallization (nucleation, growth and aggregation). The kinetics of phosphocalcic crystallization with pH=6.5 was studied starting from the artificial urine. The crystallizable solutions of the urine artificial, and three concentrations different from the tea (C1=4g.L-1, C2=16g.L-1 and C3=32g.L-1) prepared by infusion (t1=5mn, t2=10mn and t3=15mn) and by decoction were maintained with 37C°, under constant agitation (150rpm) and with the darkness, to remain close to the physiological conditions. The follow-up of the kinetics of crystallization by optical microscope with polarized light and the spectral analysis by infra-red transformed of Fourier, reveal that, in the artificial urine without inhibitor, the formed crystals with pH=6.5 are brushite. The concentration (C1=4g.L-1) of the tea has a promoter effect on the three phases of crystallization. The addition of the C2=16 g.L-1 concentration prepared by infusion during 15mn and decoction caused a reduction in the average size of the crystals and aggregates. The percentage of inhibition of the average size of the crystals and the aggregates of 63% and 84% for respectively C2 is infused during 15 minutes (t3) and by decoction. The inhibiting effect of the C3 concentration on the crystallization of brushite with pH=6.5 is clearly highlighted in all the cases of figure. Its intensity varies according to the method of preparation. The percentage of inhibition of the average size of the crystals and the aggregates reaches 47%, 69%, 89% and 99% for respectively t1, t2, t3 and decoction. Inhibition is proportional to times of infusion and it is maximum for the decoction.

KEYWORDS: green tea; urine artificial; crystallization; brushite; inhibition

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